Chidchanok Prathumwon a , Songyot Anuchapreeda b , Kanokwan Kiattisin a , Pawaret Panyajai b , Panikchar Wichayapreechar d , Young-Joon Surh e , Chadarat Ampasavate a,c,*
a Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
b Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
c Center for Excellence in Pharmaceutical Nanotechnology, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
d Department of Cosmetic Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, Thailand
e College of Pharmacy, Seoul National University, Seoul 151-741, South Korea
Corresponding author at: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
E-mail address: chadarat.a@cmu.ac.th (C. Ampasavate).
ABSTRACT
Curcumin (Cur) and epigallocatechin gallate (EGCG), the primary active compounds in turmeric and green tea, respectively, have been investigated for their anti-aging potential. The Cur and EGCG combination was encapsulated in sustained-release nanostructured lipid carriers (NLCs) to enhance their bioactivities and pharmaceutical properties. A significant enhancement in the antioxidant activities of the Cur and EGCG combination was observed at an optimal ratio, as demonstrated by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (118.83 ± 3.78 %), ferric ion reducing antioxidant power assay (217.25 ± 13.45 %), and lipid peroxidation inhibition assay (106.08 ± 12.93 %), compared to Cur alone without compromising the antioxidant activities and total phenolic content of EGCG. This is due to the enhancement of total phenolic content of the combination of 218.83 ± 10.57 %. For anti-aging activities, the combination exhibited stimulation of SIRT1 protein and inhibition of collagenase and elastase of 27.53 ± 0.73 %, 43.70 ± 1.05 % and 51.76 ± 6.52 % compared with that achieved with Cur alone, respectively. The incorporation of the Cur and EGCG combination into NLCs resulted in high entrapment efficiencies of 98.60 ± 0.05 % for Cur and 98.40 ± 0.08 % for EGCG, with corresponding loading capacities of 0.789 ± 0.001 % and 3.935 ± 0.003 %, respectively. When formulated NLCs into an emulgel base, the system demonstrated sustained release profiles over 48 h, with 12.82 ± 0.99 % release of Cur and 63.77 ± 5.76 % release of EGCG. Significant skin retention was also observed after 24 h, with 23.88 ± 1.71 % Cur and 22.79 ± 4.65 % EGCG retained in the skin. Therefore, Cur: EGCG-loaded NLCs in emulgel can deliver the active compounds into the dermis, enhancing skin penetration, sustained delivery, and anti-aging activity superior to each conventional single active compound in topical formulations.
ARTICLE INFO
Keywords: Curcumin Epigallocatechin gallate SIRT1 Anti-aging HaCaT cells Nanostructured lipid carriers Skin
Tarik Zahra,b,y , Vijay K. Bodac,d,y , Jian Gee,f,y , Lexiang Yua,g , Zhongzhi Wuc,d , Jianwen Quee,f,h, *, Wei Lic,d, *, Li Qianga,g, *
a Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA
b Department of Molecular Pharmacology and Therapeutics, Columbia University, New York, NY 10032, USA
c Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA
d Drug Discovery Center, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA
e Division of Digestive and Liver Diseases, Columbia University, New York, NY 10032, USA
f Center for Human Development, Columbia University, New York, NY 10027, USA
g Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
h Department of Medicine, Columbia University, New York, NY 10032, USA
*Corresponding authors.
E-mail addresses: jq2240@cumc.columbia.edu (Jianwen Que), wli@uthsc.edu (Wei Li), lq2123@cumc.columbia.edu (Li Qiang).
These authors made equal contributions to this work.
Peer review under the responsibility of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences.
Received 22 October 2023; received in revised form 13 December 2023; accepted 5 January 2024
KEY WORDS Estrogen receptor b; Aging; Metabolism; Skin injury; Muscle metabolism; Small molecule conjugates; Regeneration; Adiposity
伤口、造口、失禁护理俱乐部
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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