伤口世界

伤口世界

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Acheta domesticus: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications

Kankanit Yeerong 1 , Panuwan Chantawannakul 2 , Songyot Anuchapreeda 3,4 , Thomas Rades 5 ,

Anette Müllertz 5,6 and Wantida Chaiyana 1,4,7,*

1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

2 Bee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

3 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

4 Center of Excellence in Pharmaceutical Nanotechnology, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand

5 Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (T.R.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.M.)

6 Bioneer:FARMA, Department of Pharmacy, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark

7 Multidisciplinary and Interdisciplinary School, Chiang Mai University, Chiang Mai 50200, Thailand *

Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Abstract: Acheta domesticus is an edible insect, rich in nutritional value and considered a sustainable protein source. This study aimed to investigate the potential application of A. domesticus extracts for anti-skin-aging purposes. The extracts were prepared by maceration at ambient temperature with 95% ethanol or hexane and maceration in gentle heat (45 C) with 95% v/v ethanol or DI water. The extracts were examined for total protein, phenolic, and flavonoid contents. Protein molecular weight distribution was analyzed. The safety of the extracts was investigated in terms of irritation and cytotoxicity. Biological activities relevant to the inhibition of skin aging were evaluated, including increasing transforming growth factor-beta 1 (TGF-β1) expression and inhibitory activities on collagenase and hyaluronidase. The aqueous extract from maceration in gentle heat had the highest total protein content (63 ± 1% w/w), total phenolic content (0.48 ± 0.03 mg GAE/g extract), TGF-β1

stimulating activities (33 ± 2 pg/mL), and collagenase inhibition (with a half maximal inhibitory

concentration of 26 ± 1 µg/mL) among various extracts investigated. It caused no irritation to the

hen’s egg chorioallantoic membrane and showed no cytotoxicity to human dermal fibroblasts and

peripheral blood mononuclear cells. Therefore, aqueous A. domesticus extract is proposed as an

innovative natural anti-skin-aging ingredient.

Keywords: Acheta domesticus; anti-aging skincare; cosmetic; human dermal fibroblast; safety

Protective Effects of Sesame Glycoproteins on Ultraviolet-Induced Skin Aging: In Vitro and In Vivo Studies

Kyung Kyu Baik 1,†, Woo-Yong Song 2,†, Dong Keun Song 1 , Jaehyeok Yun 1 , Ji Hwan Jang 1 , Jae Young Oh 1 Mi-Jin Lee 2 , Eunjandi Go 2 , Kyong Jin Lee 2 , Eunmiri Roh and Jong-Eun Kim 1,*

1 Department of Food Science and Technology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (K.K.B.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (D.K.S.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.Y.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.H.J.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.Y.O.)

2 CNABIOTECH Co., Ltd., Cheongju-si 28106, Republic of Korea; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (W.-Y.S.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.-J.L.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (E.G.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (K.J.L.)

3 Department of Cosmetic Science, Kwangju Women’s University, Gwangju 62396, Republic of Korea; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 

Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。; Tel.: +82-43-820-5245; Fax: +82-43-820-5240

† These authors contributed equally to this work

Abstract: Background/Objectives: Ultraviolet (UV) radiation is a primary factor in skin photoaging, leading to wrinkles, reduced elasticity, and pigmentation changes due to damage to cellular DNA, proteins, and lipids. Glycoproteins from sesame cake (SPE) have potential protective effects against UV-induced skin aging. This study investigated the anti-photoaging effects of SPE on UV-induced damage in human keratinocyte HaCaT cells and SKH-1 hairless mice. Methods: SPE was evaluated for its ability to mitigate UV-induced damage in HaCaT cells by assessing MMP-1 protein and mRNA expression levels, as well as the activity of transcription factors AP-1 and NF-κB. The phosphorylation of AKT and MAPK pathways was also analyzed. In vivo, SKH-1 hairless mice were exposed to UV radiation, and the effects of SPE on wrinkle formation and skin structure were assessed by measuring wrinkle length, area, and volume. Results: SPE significantly inhibited UV-induced MMP-1 protein and mRNA expression in HaCaT cells, indicating suppression of AP-1 and NF-κB transcription factors involved in MMP-1 production. Additionally, SPE reduced UV-induced phosphorylation of AKT and MAPK pathways. In SKH-1 hairless mice, SPE treatment led to significant reductions in wrinkle length, area, and volume, preserving skin structure in UV-exposed mice. Conclusions: The findings demonstrate that SPE has protective effects against UV-induced photoaging by inhibiting key molecular pathways associated with skin aging. SPE shows promise as a natural anti-photoaging agent, providing a foundation for future skincare product development. Further studies are warranted to explore the molecular mechanisms in detail and to validate these effects through clinical trials.

Keywords: sesame glycoproteins; ultraviolet; skin; aging

Effects of Hypochlorous Acid Solutions on Venous Leg Ulcers (VLU): Experience With 1249 VLUs in 897 Patients

Cheryl M. Bongiovanni, PhD, RVT, CWS, FASA, FACCWSa,b, *

a Wound Clinics and Vascular Laboratories, Lake Health District, Lake District Hospital, 700 South J Street, Lakeview, OR 97630, USA; and

b Lake Wound Clinic – Klamath Falls, LLC, 2301 B Mountain View Boulevard, Klamath Falls, OR 97601, USA

KEYWORDS: Venous leg ulcer (VLU); Hypochlorous acid; Multidrug resistant infection

Betaine–salicylic acid cocrystal for enhanced skincare and acne treatment†

Zhenyuan Wang,ab Mi Wang, *a Qingsheng Tao,c Yufei Li, d Hao Wang,a Mei Zhang,c Xueli Liuc and Jiaheng Zhang *a

a Sauvage Laboratory for Smart Materials, School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China. E-mail: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。, 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

b Shenzhen Shinehigh Innovation Technology Co., Ltd., Shenzhen 518055, China

c Advanced Research, L'Oreal Research & Innovation China, Shanghai 201206, China

d The Centre in Artificial Intelligence Driven Drug Discovery, Faculty of Applied Sciences, Macao Polytechnic University, Macao 999078, China

† Electronic supplementary information (ESI) available. CCDC 2101906. For ESI

and crystallographic data in CIF or other electronic format see DOI: https://doi.

org/10.1039/d5md00001g

Salicylic acid (SA) is a natural lipophilic active ingredient commonly used in cosmetics and skin disease treatments, offering benefits such as exfoliation, anti-inflammation effects, antibacterial properties, oil control, and acne alleviation. However, its poor water solubility, low bioavailability, and potential side effects, such as allergies, irritation, and dryness, hinder its widespread application. In this study, we prepared a betaine–salicylic acid (BeSA) cocrystal and systematically characterized its crystal structure, biological activity, and clinical efficacy. The results showed that BeSA has significantly lower irritancy and cytotoxicity than SA, but exhibits excellent anti-inflammatory and antioxidant properties as well as high moisturizing and anti-acne efficacy, making it a potential alternative to SA. Further, quantum chemical calculations and molecular docking simulations were conducted to investigate the intrinsic mechanisms underlying the excellent bioactivity of BeSA cocrystals. This study introduces an innovative solution for safer and more effective skincare formulations based on SA and offers theoretical guidance regarding material engineering and further material optimization, which has crucial implications for both industry and academia.

Received 1st January 2025,

Accepted 4th February 2025

DOI: 10.1039/d5md00001g rsc.li/medchem

Treatment effects of two pharmaceutical skin care creams for xerotic feet among persons with diabetes: Rationale and design of a two-armed double blind randomized controlled trial

Anna Ericsson a,* , Karin Borgstrom¨ b,c , Christine Kumlien a,c,d , Magdalena Gershater Annersten a,c , Tautgirdas Ruzgas b,c , Johan Engblom b,c , Petri Gudmundsson b,c , Victoria Lazer a , Skaidre Jankovskaja b,c , Eva Lavant b,c , Sophia Ågren-Witteschus d , Sebastian Bjorklund ¨ b,d , Saman Salim e,f , Mikael Åstrom¨ g , Stefan Acosta f

Department of Care Science, Faculty of Health and Society, Malm¨ o University, Malm¨ o, Sweden

Department of Biomedical Science, Faculty of Health and Society, Malm¨ o University, Malm¨ o, Sweden

Biofilms Research Center for Biointerfaces, Malm¨ o University, Malm¨ o, Sweden

Department of Cardiothoracic and Vascular Surgery, Skåne University Hospital, Malm¨ o, Sweden

Department of Dermatology and Allergy Centre, Odense University Hospital, Odense, Denmark

Department of Clinical Sciences, Lund University, Malmo, ¨ Sweden

StatCons, Sweden

ARTICLE INFO

Keywords: Diabetes mellitus   Dry feet  Prevention  Foot-xerosis  Self-care

ABSTRACT

Introduction: To minimize the risk of developing foot-ulcers, persons with diabetes are given the advice to daily inspect their feet and to apply skincare formulations. However, commercially available skincare products have rarely been developed and evaluated for diabetes foot care specifically. The primary aim of this randomized controlled trial (RCT) is to evaluate the effects in reducing foot xerosis in persons with diabetes without footulcers using two skincare creams containing different humectants (interventions) against a cream base nonhumectant (comparator). Secondary outcomes are to evaluate differences on skin barrier integrity, lowmolecular weight biomarkers and skin microbiota, microcirculation including transcutaneous oxygen pressure, degree of neuropathy, and HbA1c between intervention-comparator creams.

Methods: Two-armed double-blind RCT, registered in ClinicalTrials.gov Identifier: NCT06427889. With 80 % power, two-tailed significance of 2.5 % in each arm, 39 study persons is needed in each arm, total 78 persons, 98 including dropouts, to be able to prove a reduction of at least one category in the Xerosis Severity Scale with the intervention creams compared to the comparator. In one arm, each participant will treat one foot with one of the intervention creams (Oviderm® or Canoderm®), while the opposite foot will be treated with the comparator cream (Decubal®lipid cream), twice a day. If needed, participants are enrolled after a wash-out period of two weeks. The participants will undergo examinations at baseline, day 14 and day 28.

Discussion: This RCT evaluate the potential effects of humectants in skin creams against foot xerosis in persons with diabetes.

Enhancement of skin regeneration through activation of TGF-β/SMAD signaling pathway by Panax ginseng meyer non-edible callus-derived extracellular vesicles

Ha Young Park a , Min Ho Kang a , Guewha Lee b , Jin Woo Kim a,c,*

Department of Food Science, Sunmoon University, Chungcheongnam-do, Republic of Korea

Hu evergreen Pharm Inc., Incheon, Republic of Korea

Center for Next-Generation Semiconductor Technology, Sun Moon University, Chungnam, Republic of Korea

ARTICLE INFO

Keywords: Ginseng non-edible callus  Extracellular vesicle  Skin regeneration  Collagen synthesis  Proliferation

1 . ABSTRACT

Background: This study aimed to investigate the effects of ginseng non-edible callus-derived extracellular vesicle (GNEV) on skin regeneration, particularly focusing on its impact on proliferation and migration in human dermal fibroblast (HDF).

Methods: GNEV was isolated from ginseng non-edible callus using sequential filtration and size exclusion chromatography (SEC). The extracellular vesicle was characterized using nanoparticle tracking analysis (NTA). HDF was treated with various concentrations of GNEV, and cell viability, proliferation, and migration were assessed using MTT and scratch wound healing assays. Gene expression related to collagen synthesis (TGF-β, SMAD-2, SMAD-3, COL1A1) was measured using RT-PCR.

Results: Treatment of HDF with GNEV resulted in a significant 2.5-fold increase in cell migration compared to the non-treated group. Furthermore, GNEV demonstrated the upregulation of collagen synthesis genes, specifically TGF-β, SMAD-2, SMAD-3, and COL1A1, by 41.7 %, 59.4 %, 60.2 %, and 21.8 %, respectively. These findings indicated that GNEV activates the TGF-β/SMAD signaling pathway, showcasing its potential to induce skin

Conclusions: In conclusion, GNEV exhibits a notable ability to enhance skin regeneration through its stimulatory effects on cell migration and the upregulation of key collagen synthesis genes. The activation of the TGF-β/SMAD signaling pathway further suggests the potential of GNEV as a promising candidate for drug delivery systems in the fields of cosmetics and pharmaceuticals, opening avenues for further research and application in skincare and dermatology