Investigation of the anti-aging effects of active components of Artemia franciscana loaded in hyalurosome

Mona Sadat Mirtaleb1,2, Mahvash Khodabandeh Shahraky1, Abdolkhaleg Deezagi3 & Danial Shafiee1

1 Department of Bioprocess Engineering, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

2 Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran. 3Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology

(NIGEB), Tehran, Iran. email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

With the advancement of biotechnology in the marine industry, an increasing utilization of marine ingredients in skincare products has been observed in recent years. Encapsulating Artemia franciscana extract and its derivatives in a novel phospholipid vesicle called hyalurosome presents innovative strategies for drug delivery systems and anti-aging products. In this study, we developed nano hyalurosomes containing Artemia franciscana active components. Partially purification of proteins in the Artemia franciscana extract was performed using ion exchange chromatography, specifically targeting Hsp40 and Artemin. The physicochemical properties of the hyalurosomes were characterized, revealing nanoparticle sizes ranging from 100 to 130 nm, zeta potential between −57 and −41.2 mV. The biological compatibility of the fabricated hyalurosomes was tested in vitro on mice fibroblast cells. Results indicated that formulations containing hyalurosomes exhibited no cytotoxicity. In-vivo studies employing H&E and Mason’s trichrome staining demonstrated an increase in the dermal layer of the skin on male mice and collagen production following treatment with different formulations containing hyalurosomes. Therefore, these formulations are considered promising candidates for anti-aging effects. Stability study at 4 °C for 60 days validated by FE-SEM imaging. In conclusion, hyalurosomes fabricated with Artemia franciscana extract and its diverse active molecules successfully achieved enhanced loading and penetration into the deeper layers of the skin, and it can be a suitable candidate for the treatment of skin aging and rejuvenation.

Keywords Nano drug delivery, Hyalurosome, Marine biotechnology, Artemia franciscana, Skin regeneration

Aromatic plants as cosmeceuticals: benefts and applications for skin health

Jesus Olivero‑Verbel1  · Patricia Quintero‑Rincón2,3 · Karina Caballero‑Gallardo1,2

Received: 25 June 2024 / Accepted: 9 October 2024 / Published online: 5 November 2024

© The Author(s) 2024

Abstract

Main conclusion This review highlights the potential of aromatic plants as natural antioxidants in cosmeceuticals to combat skin aging and promote health and rejuvenation.

Abstract Aromatic plant extracts, essential oils, or their phytoconstituents have a long history of use in skincare, dating back centuries. Currently, these plant-based sources are extensively researched and utilized in the cosmeceutical industry to formulate products that enhance skin health and promote a youthful appearance. These plants’ diverse bioactivities and sensory properties make them ideal ingredients for developing anti-aging agents recommended for maintaining healthy skin through self-care routines, offering a natural alternative to synthetic products. Reactive oxygen species (ROS) accumulation in the dermis, attributed to intrinsic and extrinsic aging factors, particularly prolonged sun exposure, is identified as the primary cause of skin aging. Plant extracts enriched with antioxidant compounds including favonoids, phenolics, tannins, stilbenes, terpenes, and steroids, are fundamental to counteract ROS-induced oxidative stress. Noteworthy effects observed from the use of these natural sources include photoprotective, senolytic, anti-infammatory, anti-wrinkle, anti-acne, and antityrosinase activities, encompassing benefits like photoprotection, wound healing, skin whitening, anti-pigmentation, tissue regeneration, among others. This review highlights several globally distributed aromatic plant species renowned for their benefits for skin, including Foeniculum vulgare Mill. (Apiaceae), Calendula ofcinalis L. and Matricaria chamomilla L. (Asteraceae), Thymus vulgaris L. (Lamiaceae), Litsea cubeba (Lour.) Pers. (Lauraceae), Althaea ofcinalis L. (Malvaceae), Malaleuca alternifolia (Maiden y Betche) Cheel (Myrtaceae), Cymbopogon citratus (DC.) Stapf (Poaceae), Rubus idaeus L. (Rosaceae), and Citrus sinensis L. Osbeck (Rutaceae), emphasizing their potential in skincare formulations and their role in promoting health and rejuvenation.

Keywords Cosmeceutical · Bioactive compounds · Antioxidants · Anti-aging · Antiphotoaging · Aromatic plants

Changes in human skin composition due to intrinsic aging: a histologic and morphometric study

Marta Arnal‑Forné1  · Tamara Molina‑García2  · María Ortega2  · Víctor Marcos‑Garcés2,3 · Pilar Molina4  · Antonio Ferrández‑Izquierdo1,2,5 · Pilar Sepulveda1,6,7 · Vicente Bodí2,3,6,8 · César Ríos‑Navarro1,2,6 · Amparo Ruiz‑Saurí1,2,6

César Ríos-Navarro

该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

* Amparo Ruiz-Saurí

该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

1 Department of Pathology, University of Valencia, Avda. Blasco Ibáñez 15. 46010, Valencia, Spain

2 Instituto de Investigación Sanitaria INCLIVA Biomedical Research Institute, Avda. Menéndez Pelayo 4acc, 46010 Valencia, Spain

3 Cardiology Department, Hospital Clínico Universitario, Valencia, Spain

4 Department of Pathology, Instituto de Medicina Legal y Ciencias Forenses, Valencia, Spain

5 Anatomic Pathology Department, Hospital Clínico Universitario, Valencia, Spain

6 Centro de Investigación Biomédica en Red (CIBER)-CV, Madrid, Spain

7 Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain

8 Department of Medicine, University of Valencia, Valencia, Spain

Accepted: 10 June 2024 / Published online: 2 July 2024 © The Author(s) 2024

Abstract

Skin represents the main barrier against the external environment, but also plays a role in human relations, as one of the prime determinants of beauty, resulting in a high consumer demand for skincare-related pharmaceutical products. Given the importance of skin aging in both medical and social spheres, the present research aims to characterize microscopic changes in human skin composition due to intrinsic aging (as opposed to aging infuenced by external factors) via histological analysis of a photoprotected body region. Samples from 25 autopsies were taken from the periumbilical area and classifed into four age groups: group 1 (0–12 years), group 2 (13–25 years), group 3 (26–54 years), and group 4 (≥55 years). Diferent traditional histological (hematoxylin–eosin, Masson’s trichrome, orcein, toluidine, Alcian blue, and Feulgen reaction) and immunohistochemical (CK20, CD1a, Ki67, and CD31) stains were performed. A total of 1879 images photographed with a Leica DM3000 optical microscope were morphometrically analyzed using Image ProPlus 7.0 for further statistical analysis with GraphPad 9.0. Our results showed a reduction in epidermis thickness, interdigitation and mitotic indexes, while melanocyte count was raised. Papillary but not reticular dermis showed increased thickness with aging. Specifcally, in the papillary layer mast cells and glycosaminoglycans were expanded, whereas the reticular dermis displayed a diminution in glycosaminoglycans and elastic fbers. Moreover, total cellularity and vascularization of both dermises were diminished with aging. This morphometric analysis of photoprotected areas reveals that intrinsic aging signifcantly infuences human skin composition. This study paves the way for further research into the molecular basis underpinning these alterations, and into potential antiaging strategies.

Keywords Skin aging · Intrinsic aging · Morphometric analysis · Human biopsies

Noninvasive Ocular Surface Workup in Patients with Meibomian Gland Dysfunction Using Microwave-Heated Eye Bag

Costanza Rossi1, *, Sabrina Vaccaro1, *, Massimiliano Borselli1 , Giovanna Carnovale Scalzo1 , Mario Damiano Toro2, Vincenzo Scorcia1 , Giuseppe Giannaccare 31

Department of Ophthalmology, University Magna Graecia of Catanzaro, Catanzaro, Italy; 2 Eye Clinic, Public Health Department, University of Naples

Federico II, Naples, Italy; 3 Eye Clinic, Department of Surgical Sciences, University of Cagliari, Cagliari, Italy

*These authors contributed equally to this work

Correspondence: Giuseppe Giannaccare, Ophthalmology, Chairman of Eye Clinic, Department of Surgical Sciences, University of Cagliari, Cagliari, Italy, Email 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Purpose: To report the outcomes of a novel microwave heating device (Blepha EyeBag®) used serially for the treatment of meibomian gland dysfunction (MGD).

Patients and Methods: This prospective single center study was conducted at University Magna Graecia of Catanzaro. Patients were instructed to apply the compress twice daily for 15 days and once per day every two days, as reported in the package insert. Outcome measures were i) ocular surface disease index (OSDI) score, ii) tear meniscus height (TMH), iii) non-invasive keratograph break-up time (NIKBUT) (first and average), iv) meiboscore, v) bulbar redness. Evaluations were performed at baseline (T0) after 15 days (T1) and after 45 days of therapy (T2).

Results: Overall, 19 patients with MGD (8 males, 11 females; mean age 64.58 ± 9.72 years) were included. The mean value of OSDI score showed a significant decrease from 28.16 ± 17.46 at T0 to 13.69 ± 7.62 at T2 (p=0.008). The mean value of NIKBUT first significantly increased from 6.67 ± 3.51 seconds (s) at T0 to 10.46 ± 4.64 at T2 (p=0.0121); in parallel, the mean value of NIKBUT average increased significantly from 11.09 ± 4.15 s at T0 to 14.95 ± 4.85 at T2 (p=0.0049). No significant differences were detected at each time point for bulbar redness, meiboscore and TMH. Throughout the entire study, no adverse events were recorded.

Conclusion: The microwave-heated eye bag treatment is both safe and effective for treatment of MGD, being able to significantly ameliorate both patient-reported symptoms and tear film stability.

Keywords: MGD, dry eye, ocular surface disease, warming device, eyelid

Integrative Multiomics Approach to Skin: The Sinergy between Individualised Medicine and Futuristic Precision Skin Care?

Angelica Dessì, Roberta Pintus, Vassilios Fanos and Alice Bosco *

Neonatal Intensive Care Unit, Department of Surgical Sciences, University of Cagliari, AOU Cagliari, 09124 Cagliari, Italy; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.D.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (R.P.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (V.F.) * Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Citation: Dessì, A.; Pintus, R.; Fanos, V.; Bosco, A. Integrative Multiomics Approach to Skin: The Sinergy between Individualised Medicine and Futuristic Precision Skin Care?

Metabolites 2024, 14, 157. https:// doi.org/10.3390/metabo14030157

Academic Editors: Eleftherios Panteris and Olga Deda

Received: 1 February 2024

Revised: 1 March 2024

Accepted: 4 March 2024

Published: 7 March 2024

Abstract: The skin is a complex ecosystem colonized by millions of microorganisms, the skin microbiota, which are crucial in regulating not only the physiological functions of the skin but also the metabolic changes underlying the onset of skin diseases. The high microbial colonization together with a low diversity at the phylum level and a high diversity at the species level of the skin is very similar to that of the gastrointestinal tract. Moreover, there is an important communication pathway along the gut–brain–skin axis, especially associated with the modulation of neurotransmitters by the microbiota. Therefore, it is evident that the high complexity of the skin system, due not only to the genetics of the host but also to the interaction of the host with resident microbes and between microbe and microbe, requires a multi-omics approach to be deeply understood. Therefore, an integrated analysis, with high-throughput technologies, of the consequences of microbial interaction with the host through the study of gene expression (genomics and metagenomics), transcription (transcriptomics and meta-transcriptomics), and protein production (proteomics and meta-proteomics) and metabolite formation (metabolomics and lipidomics) would be useful. Although to date very few studies have integrated skin metabolomics data with at least one other ‘omics’ technology, in the future, this approach will be able to provide simple and fast tests that can be routinely applied in both clinical and cosmetic settings for the identification of numerous skin diseases and conditions. It will also be possible to create large archives of multi-omics data that can predict individual responses to pharmacological treatments and the efficacy of different cosmetic products on individual subjects by means of specific allotypes, with a view to increasingly tailor-made medicine. In this review, after analyzing the complexity of the skin ecosystem, we have highlighted the usefulness of this emerging integrated omics approach for the analysis of skin problems, starting with one of the latest ‘omics’ sciences, metabolomics, which can photograph the expression of the genome during its interaction with the environment.

Keywords: skinomics; multiomics; precision skin care; skin metabolome; system biology

Lemon Balm (Melissa officinalis L.) Leaf Extract Promotes Endo180 Production in Dermal Fibroblasts and has Antiwrinkle Effect on Human Skin

Hiroyasu Iwahashi1 | Yoshihito Kawashima1 | Hitoshi Masaki2,3 | Atsushi Taga4,5

1Research Center, Maruzen Pharmaceuticals Co., Ltd., Hiroshima, Japan | 2Laboratory of Photoaging Research, School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo, Japan | 3Research Institute for Human Health Science, Konan University, Hyougo, Japan | 4Pathological and Biomolecule Analyses Laboratory, Faculty of Pharmacy, Kindai University, Osaka, Japan | 5Antiaging Center, Kindai University, Osaka, Japan

Correspondence: Hiroyasu Iwahashi (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。)

Received: 7 June 2024 | Revised: 7 January 2025 | Accepted: 7 January 2025

Funding: The authors received no specific funding for this work.

Keywords: Endo180 | Melissa officinalis | photoaging | type I collagen | wrinkle