CASIN exerts anti-aging effects through RPL4 on the skin of naturally aging mice

Yijia Zhang1 | Xueer Wang1 | Jianyuan Huang1 | Xinyue Zhang1 | Lingwei Bu1 |

Yarui Zhang1 | Fengting Liang1 | Shenhua Wu1 | Min Zhang1 | Lu Zhang2 |

Lin Zhang1

1 GDMPA Key Laboratory of key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China

2 Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Center for Orthopaedic Surgery of the Third Affiliated Hospital, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Southern Medical University, Guangzhou, China

Correspondence

Lu Zhang, Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Center for Orthopaedic Surgery of the Third Affiliated Hospital, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Southern Medical University, Guangzhou, China.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 Lin Zhang, GDMPA Key Laboratory of key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

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Funding information

National Natural Science Foundation of China, Grant/Award Number: 82073417, 81872514 and 81971297; GDMPA Key Laboratory Project of Scientific and Technological Innovation, Grant/Award

Number: 2023ZDZ12; Guangdong Basic and Applied Basic Research Foundation, Grant/Award Number: 2022A1515010768, 2023A1515012480 and 2024A121301233

Abstract

Skin aging has been associated with the onset of various skin issues, and recent studies have identified an increase in Cdc42 activity in naturally aging mice. While previous literature has suggested that CASIN, a specific inhibitor of Cdc42 activity, may possess anti-aging properties, its specific effects on the epidermis and dermis, as well as the underlying mechanisms in naturally aging mice, remain unclear. Our study revealed that CASIN demonstrated the ability to increase epidermal and dermal thickness, enhance dermal-epidermal junction, and stimulate collagen and elastic fiber synthesis in 9-, 15-, and 24-month-old C57BL/6 mice in vivo. Moreover, CASIN was found to enhance the proliferation, differentiation, and colony formation and restore the cytoskeletal morphology of primary keratinocytes in naturally aging skin in vitro. Furthermore, the anti-aging properties of CASIN on primary fibroblasts in aging mice were mediated by the ribosomal protein RPL4 using proteomic sequencing, influencing collagen synthesis and cytoskeletal morphology both in vitro and in vivo. Meanwhile, both subcutaneous injection and topical application exhibited anti-aging effects for a duration of 21 days. Additionally, CASIN exhibited anti-inflammatory properties, while reduced expression of RPL4 was associated with increased inflammation in the skin of naturally aging mice. Taken together, our results unveil a novel function of RPL4 in skin aging, providing a foundational basis for future investigations into ribosomal proteins. And CASIN shows promise as a potential anti-aging agent for naturally aging mouse skin, suggesting potential applications in the field.

KEYWORDS

CASIN, Cdc42, fibroblast, keratinocyte, ribosome, skin aging

Facial assessment methods for inhibiting glycation and aging effects and the correlation between glycation and aging parameters

Qin Zhang1, Dangdang Cheng1,2 & FeifeiWang3

This study aimed to develop in vivo methods for assessing facial anti-glycation and anti-aging effects and to investigate the link between glycation and aging signs. We utilized an AGE reader to measure AGEs levels on the face and arms, establishing a correlation to validate the reader’s use for facial AGEs detection. Then the product’s 7-day anti-glycation effect was evaluated. And its 56-day anti-aging effects were evaluated using non-invasive probes and VISIA CR, assessing skin tone (skin brightness L* and yellowness b*, skin gloss), texture (skin elasticity R2 and firmness F4, skin pores), and hydration (skin moisture content). Correlations between facial AGEs levels and aging parameters were analyzed.

Results indicated a strong correlation between facial and arm AGEs after product application, supporting facial AGEs level as an assessment parameter. The product demonstrated substantial antiglycation and anti-aging effects, suggesting the methods’ efficacy for cosmetic evaluation. A stronger overall correlation was found between AGEs levels and skin tone than with skin texture and hydration, highlighting glycation’s impact on skin color.

Keywords AGE reader, Anti-glycation evaluation, Anti-aging evaluation, Glycation-aging correlation

Senomorphic activity of a combination of niacinamide and hyaluronic acid: correlation with clinical improvement of skin aging

Patrick Bogdanowicz 1,4囚 , Paul Bensadoun 2,4, Maïté Noizet 1 , Benoît Béganton 1 , Armony Philippe 1 , SandrineAlvarez‑Georges 1 , Gautier Doat 3 , AmélieTourette 1 , Sandrine Bessou‑Touya 1 , Jean‑Marc Lemaitre 2囚 & Hélène Duplan 1

1 R&D Pierre Fabre Dermo-Cosmétique & Personal Care, Toulouse, France.

2 INSERM IRMB UMR1183, Hôpital Saint Eloi, Université de Montpellier, Montpellier, France.

3 Laboratoires Dermatologiques Avène, Lavaur, France. 4These authors contributed equally: Patrick Bogdanowicz and Paul Bensadoun.

囚email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Intrinsic and extrinsic factors, including lifestyle and sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead to skin aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) that includes MMPs, CXCLs and S100A8/9. Reducing the SASP with senotherapeutics is a promising strategy to reduce skin aging. Here we evaluated the effect of a formula containing niacinamide and hyaluronic acid, which are known to limit senescence and skin aging. We conducted three different studies. (1) Ex vivo explants treated with the formula had more collagen and glycosaminoglycan. (2) In a clinical trial with forty-four women, two months of treatment improved fne lines, wrinkles, luminosity, smoothness, homogeneity, and plumpness. (3) In a third study on thirty women, we treated one arm for two months and took skin biopsies to study gene expression. 101 mRNAs and 13 miRNAs were differentially expressed. We observed a likely senomorphic efect, as there was a decrease in many SASP genes including MMP12 and CXCL9 and a significant downregulation of autocrine signaling genes: S100A8 and S100A9. These pharmaco-clinical results are the first to demonstrate the senomorphic properties of an effective anti-aging formula in skin.

The state of the art in anti-aging: plant-based phytochemicals for skin care

Merve Tomas1 , Deniz Günal-Köroğlu1 , Senem Kamiloglu2,3, Tugba Ozdal4 and Esra Capanoglu1,5*

*Correspondence: Esra Capanoglu 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

1 Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, 34469 Maslak, Istanbul, Türkiye

2 Department of Food Engineering, Faculty of Agriculture, Bursa Uludag University, 16059 Gorukle, Bursa, Türkiye

3 Science and Technology Application and Research Center (BITUAM), Bursa Uludag University, 16059 Gorukle, Bursa, Türkiye

4 Department of Food Engineering, Faculty of Engineering and Natural Sciences, Istanbul Okan University, 34959 Tuzla, Istanbul, Türkiye

5 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

Abstract

Phytochemicals help mitigate skin aging by scavenging free radicals, modulating key enzymatic pathways, and promoting the skin’s structural integrity. Carotenoids, vitamins, essential fatty acids, and phenolic compounds work by acting as antioxidants, inhibiting enzymes like hyaluronidase, collagenase, and elastase, which degrade skin structure, and reducing levels of inflammatory markers (IL-6, IL-8, etc.) and matrix metalloproteinases (MMP-1, MMP- 2) linked to aging. Recent research highlights that plant-based phytochemicals can improve skin elasticity, reduce hyperpigmentation, prevent the breakdown of important skin proteins, and support wound healing, making them valuable components for skin care and treatments. This review explores the multifaceted roles of phytochemicals in maintaining and improving skin health, highlighting their mechanisms of action and potential in skin anti-aging

Keywords Skin care, Anti-aging, Phytochemicals, Polyphenols, Carotenoids, Fatty acids, Saponins, Alkoloids

次氯酸消毒液联合湿性愈合方法 用于慢性伤口疗效观察

封红伟 陈丽琴 胡绣华

DOI：10.3969/j.issn.0253-9926.2020.21.004

基金项目：上海市宝山区科学技术委员会科技创新专

项资金项目(17-E-8)

作者单位：200940 上海市第一人民医院宝山分院护理

部

【摘 要】 目的 观察次氯酸消毒液联合湿性愈合方法在慢性伤口中的效果。方法 对 2017 年 1 月至 2019 年 5 月在我院伤口门诊就诊慢性伤口患者(150 例 229 处伤口)，采用随机数字表格法分为观察组(75 例 116 处伤口)，采用次氯酸消毒液联合湿性愈合方法；对照组(75 例 113 处伤口)，采用常规湿性换药方法。比较 2 组患者创面愈合率、愈合时间、换药次数。 结果 观察组的伤口愈合率高于对照组，愈合时间、换药次数均少于对照组，差异有统计学意义(P<0．05)。结论 应用次氯酸消毒液联合湿性愈合方法优于常规湿性愈合方法。

【关键词】 次氯酸；消毒药；伤口愈合

Observation on the curative effect of hypochlorite disinfectant combined with wet healing on chronic wounds

Feng Hongwei, Chen Liqin, Hu Xiuhua．Department of Nursing， Baoshan Branch of Shanghai First People′s Hospital，Shanghai 200940， China

【Abstract】 Objective

To observe the effect of hypochlorite disinfectant combined with wet healing on chronic wounds．

Methods

From January 2017 to May 2019 229 wounds in 150 patients with chronic wounds were randomly divided into two groups： the observation group (116 wounds in 75 patients) of using combination of hypochlorite disinfectant and wet healing method and the control group (113 wounds in 75 patients) of using conventional wet healing method． Wound healing rate， healing time and dressing change times between the two groups were compared． Results The wound healing rate of the observation group was higher than that of the control group (P < 0．05)， and the healing time and dressing change times of the observation group were also lower than those of the conventional group (P< 0．05)． Conclusion

The combination of hypochlorite disinfectant and wet healing method is better than the conven

tional wet healing method．

【Key words】 Hypochlorous acid； Disinfectants； Wound healing

DOI：10.3969/j.issn.0253-9926.2020.21.004

WOUND INFECTIONINCLINICAL PRACTICEPrinciples of best practice

This article is excerpted from the INTERNATIONAL CONSENSUS UPDATE 2022 by Wound World.