高晓东',胡必杰',鲍容3

(1.复旦大学附属中山医院感染管理科2.临床微生物试验室，上海200032)

摘要:目的探讨次氯酸对皮肤软组织感染常见病原体(包括标准茁株及临床茁株)的体外杀菌效果,为临床应用提供依据。方法﹐根抛《消毒技术规范》将0.5左氏单位的临床分离茁株菌悬液与次氯酸作用1 min和10 min,然后将作用后的液体与中和剂混合中和消毒效果,普通病原体的混合液取1.0 ml进行琼脂倾注法培养,化脓性链球菌和非结核分枝杆菌的混合液取0.1 ml分别在血平板和7H10平板上进行涂布法培养;除非结核分枝杆菌培养7d外,其他病原体培莽48h后进行融落计数,计算杀A对数值。结果﹐次氯酸作用标准開株1 min或10min均可将菌量从0.5麦氏单位(相当于10⁷)降剑0;临床菌株包括化脓性链球菌、耐甲氧两林金黄色懒萄球菌(MRSA).耐碳青霉烯类的鲍氏不动朴菌(CRAB).耐碳青霉烯类的铜绿假弟胞菌(CRPA).耐碳青霉烯类的肺炎克需伯杆南(CRKB),脓肿分枝杆南、色分枝杆開和产气荚膜杆開也得到同样结果。结论﹐本试验首次系统全面检测次氛酸对皮肤软组织感染常见的病原体(包括非结核分枝杆開,厌氧掬,真南等)均有很好的体外杀南活性，可以作为感染伤口的局部爪药。

关键词:次氯酸;皮肤软组纠感染;病原体

中图分类号:R187文献标识码:A文章编号:I005 4529(2017)081714 03

Effectiveness of hypochlorous acid solution against common pathogens of skin and soft tissue infection in vitro

GAO Xiao-dong，HU Bi-jie，BAO Rong

(Zhongshan Hospital of Fudan University ，Shanghai 200032，China)

Abstract:ORJECTIVE To investigale the gertmicidal elficientey of hypochlorous acid solution sgainst cotutmotLpathogcns (Inucluding staudeurd strainus anud clinical strains) of skin atnud solt tissuc inlcetion in vitro.so as to provil:the bosis for improving the boctericidal effect.METHODSAccording to " technical standord for disinfection" ,acertain concentrotion of pothogens of clinical isolates wos treated with hypochlorous ocid solution for 1 min or 10 min,anud then the action of the solution anud neutralizer were tmuixeud, 1.0ml of the tuixlure of cotmtmot.puthogens was used lor agar pouring culure,0. 1 ml of the tuixlure ol Sireplococcus pyogenes anud nontuberulosismycobecterie were cultured using the cooting method in the blood plate and 7H10 plate.Unless the nontuberculo-sis mycohscteria was cultured for 7 d, the other pathogens were cultured for 48 h and the colonies were counted.RESULTS Treatment of hypochlorous acid solution for 1 min or 10 min could decrease the amount of bacte-ris from 0.5 Maxwell units (equivalent to 10⁷) to 0.The clinical isolstes of S. pyogenes，methicillin-resistantSta phylococcus aureus (MRSA)，carbepenem-resistant Acinetobacter baumannii(CRAR),carbapenem-resistantlP'seudomonas aeruginosa (CRPA),carhapenen-resistant Klebsiella pneumoniae(CRKB),Mycohacterium ab-scess ,Mycobacterium tuberculosis , and Clostridium per fringens also got the sxatme results,CONCLUISION Hypo-chlorous acid solution has good germicidal efficiency against common pethogens of skin and soft tissve infection imvittro,and it can be used as topical treatment for infected wounds.

Key words:Hypochlorous acid solution; Skin and soft tissue infection;Pathogens