Vitamin D and wound healing: Assessing skin barrier function and implications for chloasma treatment

Qiong Chen1 | Lin Liu2 | Yi Zhang1

1 The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shanghai, China

2 Dermatology Department, Jingfu Medical Beauty Hospital of Chunxi Road Ward, Chengdu, China

Correspondence

Yi Zhang, The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shanghai, China.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

© 2024 The Authors. International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd.

Abstract

Chloasma, which is distinguished by irregularities in the pigmentation of skin, poses substantial challenge in the field of dermatology. The regulatory influence of vitamin D on the functions of skin cells implies that it may have the capacity to effectively treat chloasma and promote wound healing. To assess the efficacy of vitamin D in chloasma treatment and its impact on the function of skin barrier during the process of wound healing. The research spanned from April 2022 to September 2023, in Shanghai, China, examined 480 individuals who had been diagnosed with chloasma. A double-blind, placebocontrolled clinical trial was utilized to evaluate effectiveness of topical vitamin D3 in treatment of chloasma. Concurrently, randomized control trial investigated the effects of ingested vitamin D3 supplements on the process of wound healing. Transepidermal water loss (TEWL), chloasma severity score changes, wound size reduction and skin hydration levels were critical performance indiators. Statistically, the severity scores of chloasma decreased significantly in the vitamin D treatment group at 3 and 6 months compared with the placebo (p < 0.05). The Vitamin D group exhibited superior wound healing outcomes, including more substantial reduction in lesion size and enhanced skin barrier function, as evidenced by increased skin hydration and decreased TEWL (p < 0.05). Vitamin D substantially mitigated the severity of chloasma and has beneficial effect on wound healing and integrity of the skin barrier. Based on the results obtained, vitamin D exhibited promise as a therapeutic intervention in the field of dermatology, specifically in treatment of chloasma and promotion of wound recovery.

KEYWORDS

chloasma treatment, dermatology, skin barrier function, vitamin D, wound healing

Key Messages The study aimed to evaluate the effectiveness of vitamin D in treating chloasma and enhancing wound healing.

Findings revealed that vitamin D significantly reduced chloasma severity and improved wound healing, as indicated by increased skin hydration and decreased transepidermal water loss.

The effect of ceramides on skin absorption by Raman spectroscopy

Song Hee Kim1 Jeonghoon Yun1,2 Woncheol Kim1 Eunsu Seon1 Chanhwi Lee1 Jaejoon Jeon1 Mid Eum Yun3 Keun Hyung Park1,3

1 Sunjin Clinical Research Center, Sunjin Beauty Science Co., Ltd., Seoul, South Korea

2 Faculty of Life Sciences, University of Toronto, Toronto, Ontario, Canada

3 R&D Center, Sunjin Beauty Science Co., Ltd., Seoul, South Korea

Correspondence

Keun Hyung Park (Director, Ph.D), Sunjin Clinical Research Center, Sunjin Beauty Science Co., Ltd., 8F, Gasan Hanwha Bizmetro, 43-14, Gasandigital 2-ro, Geumcheon-gu, Seoul, South Korea.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

© 2024 The Author(s). Skin Research and Technology published by John Wiley & Sons Ltd.

Abstract

Introduction: Ceramides are essential epidermal constituents that play a critical role in skin moisturization treatment as a raw material in cosmetics formulation. Recently, ceramides have been known to be frequently applied in various cosmetic formulations. Despite ceramide’s beneficial characteristics, academic research regarding ceramides and their skin absorption remains insufficient. Therefore, our study conducted clinical research employing Raman spectroscopy to investigate the effects of ceramides on skin absorption to enhance the understanding of ceramides’ dermatological functionality and their topical application in cosmetics science.

Materials and Methods: Twenty healthy individuals with dry skin have participated in this clinical trial. In this double-arm designed trial, the test group received an investigational product with ceramides (5000 ppm) and a control group received an investigational product without the ceramides while all other components remained identical. The subjects visited the clinical research center and acclimatized for 30 min in constant humidity and temperature for equilibrium, subsequently conducting a measurement. Before the trial, the research subject’s target site (lower arm area) was kept clean, devoid of any cosmetic administering 24 h before the trial when investigational product was topically applied.

Results: Our findings with Raman spectroscopy statistically demonstrate that skin absorption amount, speed and depth for both groups improved overall (p < 0.05) after administration of the investigational product. Notably, the test group received an investigational product with ceramides (5000 ppm) indicating superior effectiveness across all parameters compared to a control group from comparison analysis of each parameter (p < 0.05).

Conclusion: This study concludes that ceramide-containing cosmetics provide a beneficial effect on skin absorption via visual and statistical results of Raman spectroscopy

KEYWORDS

ceramides, cosmetic, Raman spectroscopy, skin absorption, skin clinical research

Radio frequency hyperthermia system for skin tightening effect by filled waveguide aperture antenna with compact metamaterials

Ikhwan Kim1,2 , Dong-Min Lee1,2 , Jae-Woo Shin1,3 ,Gyoun-Jung Lee1,3 , Eun-Seong Kim1,3 * and Nam-Young Kim1,2,3 *

1 RFIC Bio Center, Kwangwoon University, Seoul, Republic of Korea, 2 Department Electronic Engineering, Kwangwoon University, Seoul, Republic of Korea, 3 APR Device Center, Seoul, Republic of Korea

OPEN ACCESS

EDITED BY Daniele Tosi, Nazarbayev University, Kazakhstan

REVIEWED BY

Lorenzo Crocco, National Research Council (CNR), Italy Changyul Cheon,

University of Seoul, Republic of Korea

*CORRESPONDENCE Eun-Seong Kim, 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 Nam-Young Kim, 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

RECEIVED 29 January 2024

ACCEPTED 08 March 2024

PUBLISHED 28 March 2024

CITATION

Kim I, Lee D-M, Shin J-W, Lee G-J, Kim E-S and Kim N-Y (2024), Radio frequency hyperthermia system for skin tightening effect by filled waveguide aperture antenna with compact metamaterials. Front. Bioeng. Biotechnol. 12:1378084.

doi: 10.3389/fbioe.2024.1378084

COPYRIGHT

© 2024 Kim, Lee, Shin, Lee, Kim and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Radio frequency (RF) hyperthermia focuses on raising the target area temperature to a value exceeding 45°C. Collagen is stimulated when the temperature rises to 45°C at the dermal layer, resulting in skin tightening. However, most studies on RF hyperthermia have focused on tumor ablation or using electrodes to radiate an electromagnetic field, which is highly inefficient. This study proposed a noninvasive RF hyperthermia skin-tightening system with a compact metamaterial- filled waveguide aperture antenna. The proposed RF system increased the temperature by 11.6°C and 35.3°C with 20 and 80 W of 2.45 GHz RF power, respectively, within 60 s and exhibited a very focused effective area. Furthermore, a metamaterial was proposed to reduce the size of the waveguide aperture antenna and focus the electromagnetic field in the near-field region. The proposed metamaterial-filled waveguide aperture antenna was compact, measuring 10 mm × 17.4 mm, with a peak gain of 2.2 dB at 2.45 GHz. The measured hyperthermia performance indicated that the proposed RF system exhibited better power- and time-efficient hyperthermia performance than other RF hyperthermia systems in the cosmetic skin lifting commercial market. The proposed RF hyperthermia systems will be applied into a new generation of beauty cosmetic devices.

KEYWORDS RF hyperthermia, metamaterial, skin tightening, beauty device, waveguide aperture antenna

Putting the Formulation Back in Foam: Optimizing Seborrheic Dermatitis Treatment Across Diverse Hair Types

by RAJ CHOVATIYA, MD, PHD, MSCI*; MEREDITH TYREE POLASKEY, MS*; EDWARD LAIN, MD; TODD SCHLESINGER, MD;

HEATHER WOOLERY-LLLOYD, MD; PATRICK BURNETT, MD; and DIANE HANNA, DNP

*Dr. Chovatiya and Ms. Polaskey share co- rst authorship of this article. DR.Chovatiya is a Clinical Associate Professor at the Rosalind Franklin University of Chicago Medical School and is the Founder and Director of the Center for Medical Dermatology and Immunology Research in Chicago, Illinois. Ms. Polaskey is with the Department of Dermatology at Northwestern University Feinberg School of Medicine in Chicago, Illinois. Dr. Lain is with Sanova Dermatology and the Austin Institute for Clinical Research in Austin, Texas. Dr. Schlesinger is with the Dermatology and Laser Center of Charleston and Clinical Research Center of the Carolinas in Charleston, South Carolina. Dr. Woolery-Llloyd is with Dr. Phillip Frost's Department of Dermatology and Cutaneous Surgery at University of Miami's Miller School of Medicine in Miami, Florida. Drs. Hanna and Burnett are with Arcutis Biotherapeutics, Inc. in Westlake Village, California.

J Clin Aesthet Dermatol. 2024;17(5):30–33.

Seborrheic dermatitis (SD) is a chronic inflammatory skin condition that commonly involves the scalp, and thus, affects a diverse demographic with varying hair care needs. Current SD treatments are limited based on optimized formulation, e cacy, adverse events, and lack of placebo-controlled trials. A novel roflumilast foam formulation has emerged as a promising therapeutic option optimally designed for use on the scalp and other hair-bearing areas. We conducted a comprehensive assessment of beauty industry standards, con rming the foam formulation's alignment with industry guidelines and exclusion of potentially harmful ingredients. In addition, consultation with an expert dermatologist panel yielded a strong endorsement, underscoring a high level of con dence in prescribing the foam across diverse hair and skin types.

KEYWORDS: Seborrheic dermatitis

FUNDING: No funding was provided for this article.

DISCLOSURES: Drs. Hanna and Burnett are employees of Arcutis Biotherapeutics, Inc. EL has served as an investigator, advisor, consultant, and/or speaker for AbbVie, Eli Lilly and Company, Bristol Myers Squibb, Ortho Dermatologics, P zer Inc, Sano , Regeneron, Galderma, Dermavant, Incyte, UCB, and Journey. Dr. Schlesinger has served as a consultant, speaker and/ or investigator for AbbVie, Arcutis, Boehringer-Ingelheim, Bristol Myers Squibb, Cara Therapeutics, Eli Lilly and Company, Galderma, Janssen, Novartis, P zer Inc., UCB. Dr. Woolery-Llloyd has served as a consultant, speaker, and/or investigator for Ortho Dermatologics, L’Oréal, Eli Lillly and Company, Incyte, P zer Inc., Galderma, Allergan, Arcutis, Vyne, Eirion, and Regeneron Dr. Chovatiya has served as an advisory board member, consultant, and/or investigator for AbbVie, Apogee Therapeutics, Arcutis, Argenx, ASLAN Pharmaceuticals, Beiersdorf, Boehringer Ingelheim, Bristol Myers Squibb, Cara Therapeutics, Dermavant, Eli Lilly and Company, FIDE, Galderma, Genentech, Incyte, Janssen, LEO Pharma, L’Oréal, Nektar Therapeutics, Novan, Inc., Opsidio, P zer Inc., Regeneron, RAPT, Sano , and UCB. Dr. Polaskey has no conflicts of interest relevant to this article.

CORRESPONDENCE: Raj Chovatiya, MD, PhD, MSCI; Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Facial Skin Microbiome Composition and Functional Shift with Aging

Allison Garlet 1 , Valerie Andre-Frei 2 , Nicolas Del Bene 1 , Hunter James Cameron 3 , Anita Samuga 3 , Vimal Rawat 4 , Philipp Ternes 5 and Sabrina Leoty-Okombi 2,*

1 BASF Corporation, 540 White Plains Road, Tarrytown, NY 10591, USA; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.G.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (N.D.B.)

2 BASF Beauty Care Solutions, 32 Rue Saint Jean de Dieu, 69007 Lyon, France; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

3 BASF Corporation, 26 Davis Dr, Raleigh-Durham, NC 27709, USA; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.J.C.); 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (A.S.)

4 BASF SE, Speyerer Str. 2, 67117 Limburgerhof, Germany; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

5 BASF Metabolome Solutions GmbH, Tegeler Weg 33, 10589 Berlin, Germany; 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

* Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Citation: Garlet, A.; Andre-Frei, V.;

Del Bene, N.; Cameron, H.J.; Samuga,

A.; Rawat, V.; Ternes, P.; Leoty

Okombi, S. Facial Skin Microbiome

Composition and Functional Shift

with Aging. Microorganisms 2024, 12,

1021. https://doi.org/10.3390/

microorganisms12051021

Academic Editors: Paul D. Facey

and Claire Morgan

Received: 30 January 2024

Revised: 14 May 2024

Accepted: 16 May 2024

Published: 18 May 2024

Abstract: The change in the skin microbiome as individuals age is only partially known. To provide a better understanding of the impact of aging, whole-genome sequencing analysis was performed on facial skin swabs of 100 healthy female Caucasian volunteers grouped by age and wrinkle grade. Volunteers’ metadata were collected through questionnaires and non-invasive biophysical measurements. A simple model and a biological statistical model were used to show the difference in skin microbiota composition between the two age groups. Taxonomic and non-metric multidimensional scaling analysis showed that the skin microbiome was more diverse in the older group (≥55 yo). There was also a significant decrease in Actinobacteria, namely in Cutibacterium acnes, and an increase in Corynebacterium kroppenstedtii. Some Streptococcus and Staphylococcus species belonging to the Firmicutes phylum and species belonging to the Proteobacteria phylum increased. In the 18–35 yo younger group, the microbiome was characterized by a significantly higher proportion of Cutibacterium acnes and Lactobacillus, most strikingly, Lactobacillus crispatus. The functional analysis using GO terms revealed that the young group has a higher significant expression of genes involved in biological and metabolic processes and in innate skin microbiome protection. The better comprehension of age-related impacts observed will later support the investigation of skin microbiome implications in antiaging protection.

Keywords: skin aging; skin microbiome; gene ontology; diversity; L. crispatus; C. acnes; C. kroppenstedtii

Antiphotoaging effects of solvent fractions isolated from Allomyrina dichotoma larvae extract

Kyong Kim a , Eun-Young Park b , Dong-Jae Baek b , Chang-Seok Lee c , Yoon Sin Oh a,*

a Department of Food and Nutrition, Eulji University, Seongnam, South Korea

b College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Jeonnam, South Korea

c Department of Beauty and Cosmetic Science, Eulji University, Seongnam, South Korea

ARTICLE INFO

Keywords: Allomyrina dichotoma larvae Ultraviolet B Photo-aging Human dermal fibroblast  Collagen

ABSTRACT

Skin aging is affected by a variety of factors, including ultraviolet rays, oxidative stress, medications, smoking, and genetics. Among them, photo-aging accounts for about 80% of skin aging. The present study was evaluated to verify the potential of Allomyrina dichotoma larvae, which has recently been attracting attention as an edible insect, as an anti-aging substance. UVB irradiation at 100 mJ/cm2 was sufficient to induce photo-aging of fibroblasts within 24 h, which was alleviated after treatment with 70% ethanol extract of Allomyrina dichotoma larvae extract (ADLE). To obtain an extract from ADLE, which has a relatively high content of polyphenol compounds containing physiological activity, fractional solvent extraction was carried out using organic solvents such as hexane, chloroform, ethyl acetate, and butanol. Additionally, ethyl acetate and butanol fractions contributed to the inhibition of UVB-induced ROS production, cell damage, and senescence of fibroblasts. It was also confirmed that the two fractions can regulate the expression of MMP-1 and AP-1. In particular, the ethyl acetate fraction showed an excellent effect in recovering collagen decomposed by UVB. Therefore, these results suggest that ADLE has potential as a natural insect-derived biomaterial to inhibit UVB-induced photo-aging.