Microneedles: A novel clinical technology for evaluating skin characteristics

Seo Hyeong Kim1 Ji Hye Kim1 Yoon Mi Choi1 Su Min Seo1 Eun Young Jang1 Sung Jae Lee1 Suhyun Cho2 Do Hyeon Jeong3 Kwang Hoon Lee1

1 Cutis Biomedical Research Center Co. Ltd., Seoul, Republic of Korea

2 Yonsei BB Skin Clinic, Seoul, Republic of Korea

3 Raphas Co. Ltd., Seoul, Republic of Korea

Correspondence

Kwang Hoon Lee, Cutis Biomedical Research Center Co. Ltd., (07327) 5F, 97, Uisadang-daero, Yeongdeungpo-gu, Seoul, Republic of Korea.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Abstract

Background: Current methods for evaluating efficacy of cosmetics have limitations because they cannot accurately measure changes in the dermis. Skin sampling using microneedles allows identification of skin-type biomarkers, monitoring treatment for skin inflammatory diseases, and evaluating efficacy of anti-aging and anti-pigmentation products.

Materials and methods: Two studies were conducted: First, 20 participants received anti-aging treatment; second, 20 participants received anti-pigmentation treatment. Non-invasive devices measured skin aging (using high-resolution 3D-imaging in the anti-aging study) or pigmentation (using spectrophotometry in the anti-pigmentation study) at weeks 0 and 4, and adverse skin reactions were monitored. Skin samples were collected with biocompatible microneedle patches. Changes in expression of biomarkers for skin aging and pigmentation were analyzed using qRT-PCR.

Results: No adverse events were reported. In the anti-aging study, after 4 weeks, skin roughness significantly improved in 17 out of 20 participants. qRT-PCR showed significantly increased expression of skin-aging related biomarkers: PINK1 in 16/20 participants, COL1A1 in 17/20 participants, and MSN in 16/20 participants. In the anti-pigmentation study, after 4 weeks, skin lightness significantly improved in 16/20 participants. qRT-PCR showed significantly increased expression of skinpigmentation-related biomarkers: SOD1 in 15/20 participants and Vitamin D Receptor (VDR) in 15/20 participants. No significant change in TFAP2A was observed.

Conclusion: Skin sampling and mRNA analysis for biomarkers provides a novel, objective, quantitative method for measuring changes in the dermis and evaluating the efficacy of cosmetics. This approach complements existing evaluation methods and has potential application in assessing the effectiveness of medical devices, medications, cosmeceuticals, healthy foods, and beauty devices.

KEYWORDS

in vivo efficacy test, skin aging, skin biomarkers, skin pigmentation

Is Acne the Same Around the World?

by ANDREW ALEXIS, MD, MPH; JERRY TAN, MD; MARCO ROCHA, MD, PhD; DELPHINE KEROB, MD; ANN’LAURE DEMESSANT, PharmD; FATIMATA LY MD; YAN WU, MD, PhD; MUKTA SACHDEV, MD; and ICHIRO KUROKAWA, MD, PhD

Dr. Alexis is with the Department of Dermatology, Weill Cornell Medicine, New York, New York. Dr. Tan is with Western University, Windsor in Ontario, Canada. Dr. Rocha is with the Department

of Dermatology, Federal University of São Paulo in São Paulo, Brazil. Drs. Kerob and Demessant are with La Roche-Posay Laboratoire Dermatological Beauty in Paris, France. Dr. Ly is with the

Dermatology Department at the University Cheikh Anta Diop of Dakar in Dakar, Senegal, West Africa. Dr. Wu is with the Department of Dermatology, Peking University First Hospital in Beijing, China. Dr. Sachdev is with the Department of Dermatology, Manipal Hospital in Bangalore, India. Dr. Kurokawa is with the Department of Dermatology at the Meiwa Hospital in Hyogo, Japan.

J Clin Aesthet Dermatol. 2024;17(9):16–22.

Acne is a common skin disease associated with a range of sequelae. These include scarring and dyspigmentation, emotional and psychosocial disturbances, and occupational problems, in part because acne often manifests on the face, in addition to other body areas, and is highly visible. Worldwide, the prevalence of acne is estimated at 9.4 percent; it is most common in adolescents but also affects a relatively high proportion of adults. Early studies of acne epidemiology were conducted primarily in the United States and the United Kingdom. In more recent decades, data have been increasing for other areas of the world. There has also been more attention devoted to how acne may present and be managed in individuals with skin of color (i.e., the broad and diverse range of populations that self-identify as belonging to a non-White racial/ethnic group and share characteristics such as higher skin phototypes and propensity toward hyperpigmentation). This review seeks to highlight aspects of acne that may be unique to skin of color.

KEYWORDS: Acne vulgaris, skin of color, ethnicity, race

FUNDING: Funding for this study was provided by La Roche-Posay.

DISCLOSURES: Dr Alexis has served as a consultant and/or advisory board member for Leo, Novartis, Galderma Laboratories LP, Sano Regeneron, Dermavant, Unilever, Beiersdorf, Valeant, L’Oreal, Bristol Meyers Squibb, Scientis, Bausch Health, UCB, Arcutis, Janssen, Allergan, Almirall, AbbVie, and Sol-Gel. Dr Tan has received grants, honoraria, or served as a consultant for Bausch, Boots Walgreens, Cipher, Cutera, Galderma, La Roche Posay, Novartis, Pierre Fabre, and P zer. Dr Rocha has served as an advisor and/or received honoraria from Galderma, Pierre-Fabre, Eucerin, La Roche Posay, and Leo Pharma. Dr Kerob and Dr Demessant are employees of L’Oreal. Dr Ly has served as consultant and received honoraria from La Roche Posay. Pr Wu and Dr Sachdev have served consultants for L’Oreal. Dr. Kurokawa has received research grants from Aisin Corporation and served as a consultant for L’Oreal.

CORRESPONDENCE: Delphine Kerob, MD; Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Infraorbital Rejuvenation Combined with Thread-Lifting and Non-cross-linked Hyaluronic Acid Injection: A Retrospective, Case-Series Study

Zhi-Feng Liao1 • Wei Yang2 • Xin Li3 •Shi-wei Wang3 •  Fang-Cen Liu4 • Sheng-Kang Luo1

1 Department of Plastic and Reconstructive Surgery, Guangdong Second Provincial General Hospital, 466 Middle Xin Gang Road, Guangzhou City 510317, Guangdong Province, China

2 Department of Medical Cosmetology, Beijing Huaxia Medical Beauty Hospital, Beijing, China

3 Department of Medical, Imeik Technology Development Co., Ltd., Beijing, China

4 Guangzhou Yestar Medical Aesthetic Hospital, Guangzhou City, Guangdong Province, China

Received: 3 August 2023 / Accepted: 20 October 2023 / Published online: 14 November 2023 © The Author(s) 2023

Abstract

Background Infraorbital aging develops during the natural aging process. Various treatment options offer unique benefits, accompanied by diverse side effect profiles, and can be synergistically combined to optimize results. This study aimed to evaluate the efficacy of a comprehensive approach involving non-cross-linked hyaluronic acid injection and smooth absorbable PPDO (poly p-dioxanone) thread insertion for infraorbital rejuvenation.

Methods This retrospective case series study enrolled ten female patients with infraorbital aging from March 2022 to April 2023. Clinical outcomes, patient satisfaction, and adverse events were assessed at 1, 3, and 6 months

Results The median Global Aesthetic Improvement Scale scores evaluated by the operator and blinded evaluator were 1.70 ± 0.42 and 1.80 ± 0.35, respectively, at six months posttreatment. The median Allergan Infraorbital

Hollows Scale determined by the operator was 1.15 ± 0.34 at six months posttreatment, whereas the scores evaluated by the blinded evaluator were 1.15 ± 0.53. At six months after treatment, 50% of patients were satisfied, and an additional 40% reported strong satisfaction with the clinical improvement following treatment. No serious adverse events, such as infections, lumps, irregularities, Tyndall effect, hematoma, or skin necrosis, occurred during the treatment period.

Conclusions The combination of PPDO thread insertion and non-cross-linked hyaluronic acid injection yielded satisfactory and effective clinical outcomes with no occurrence of serious adverse events for infraorbital rejuvenation. We anticipate that this study will contribute to the advancement of novel treatment options for infraorbital  aging.

Level of Evidence IV

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Keywords Infraorbital rejuvenation   Hyaluronic acid injections  Thread-lifting  Infraorbital aging

Menopause and facial skin microbiomes: a pilot study revealing novel insights into their relationship

Martin Patrick Pagac*, Martin Stalder and Remo Campiche DSM-Firmenich, Perfumery and Beauty, Kaiseraugst, Switzerland

EDITED BY

Franck Carbonero, Washington State University Health Sciences Spokane, United States

REVIEWED BY

Audrey Gueniche, Loreal Research and Innovation, France Janeta Nikolovski, Independent Researcher, Princeton, United States

*CORRESPONDENCE

Martin Patrick Pagac, 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

RECEIVED 09 December 2023

ACCEPTED 15 February 2024

PUBLISHED 21 March 2024

CITATION

Pagac MP, Stalder M and Campiche R (2024), Menopause and facial skin microbiomes: a pilot study revealing novel insights into their relationship. Front. Aging 5:1353082.

doi: 10.3389/fragi.2024.1353082

COPYRIGHT

© 2024 Pagac, Stalder and Campiche. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Introduction: The human skin microbial composition is affected by age. Previous studies reported skin microbiome diversity shifts between elderly and significantly younger subjects. Some studies implied that menopausal status, which is inherently linked to age, could be associated with changes in skin microbial compositions. Nevertheless, the influence of menopausal status on skin microbiome profiles while minimizing the impact of aging-associated changes in skin parameters still needs further clarification.

Methods: We performed an observational study on healthy Caucasian female volunteers, which were grouped according to their pre- or postmenopausal status. Bacterial community structures on facial skin were analyzed using 16S rRNA gene sequencing. Cutometer® measurements were performed to evaluate aging-associated changes in facial skin biophysical properties.

Results: The relative abundance of the lipophilic Cutibacterium genus was decreased, and bacterial diversity was increased in skin samples of postmenopausal volunteers. The mean age difference between examined groups in this study was 12.4 years only. Accordingly, Cutometer ® measurements revealed no differences in aging-associated skin biophysical parameters between pre- and postmenopausal groups. Consequently, no correlation was detected between Shannon diversity and measured agedependent biomechanical properties of facial skin.

Discussion: These findings are in line with previous studies, which investigated the wide-ranging impact of chronological aging on skin microbial communities. However, this work reports for the first time a direct association between menopausal status and facial microbiomes on skin of similarly aged study participants, and hence uncouples aging-associated skin biophysical parameters, such as viscoelastic properties, from the equation. These findings open avenues for the development of microbiome-targeting strategies for treatment of menopause-associated skin disorders.

KEYWORDS menopause, aging, skin, microbiome, bacterial diversity, Cutibacterium

Efficacy of probiotics in hair growth and dandruff control: A systematic review and meta-analysis

Chang-Shik Yin a,1 , Trang Thi Minh Nguyen b,1 , Eun-Ji Yi b,c , Shengdao Zheng b,c , Arce Defeo Bellere b , Qiwen Zheng b , Xiangji Jin d , Myeongju Kim b , Sejic Park b , Sarang Oh b,c , Tae-Hoo Yi b,

a Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea

b Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin, Republic of Korea

c Snowwhitefactory Co, Ltd., 807 Nonhyeon-ro, Gangnam-gu, Seoul, 06032, Republic of Korea

d Department of Pharmacology, School of Medicine, Kyung Hee University, 23 Kyungheedae-ro, Dong-daemun, Seoul, Republic of Korea

ABSTRACT

Background: Probiotics are intellectually rewarding for the discovery of their potential as a source of functional food. Investigating the economic and beauty sector dynamics, this study conducted a comprehensive review of scholarly articles to evaluate the capacity of probiotics to promote hair growth and manage dandruff.

Methods: We used the PRISMA 2020 with Embase, Pubmed, ClinicalTrials.gov, Scopus, and ICTRP databases to investigate studies till May 2023. Meta-analyses utilizing the random effects model were used with odds ratios (OR) and standardized mean differences (SMD).

Result: Meta-analysis comprised eight randomized clinical trials and preclinical studies. Hair growth analysis found a non-significant improvement in hair count (SMD = 0.32, 95 % CI -0.10 to 0.75) and a significant effect on thickness (SMD = 0.92, 95 % CI 0.47 to 1.36). In preclinical studies, probiotics significantly induced hair follicle count (SMD = 3.24, 95 % CI 0.65 to 5.82) and skin thickness (SMD = 2.32, 95 % CI 0.47 to 4.17). VEGF levels increased significantly (SMD = 2.97, 95 % CI 0.80 to 5.13), while IGF-1 showed a non-significant inducement (SMD = 0.53, 95% CI -4.40 to 5.45). For dandruff control, two studies demonstrated non-significant improvement in adherent dandruff (OR = 1.31, 95 % CI 0.13–13.65) and a significant increase in free dandruff (OR = 5.39, 95 % CI 1.50–19.43). Hair follicle count, VEGF, IGF-1, and adherent dandruff parameters were recorded with high heterogeneity. For the systematic review, probiotics have shown potential in improving hair growth and controlling dandruff through modulation of the immune pathway and gut-hair axis. The Wnt/β-catenin pathway, IGF-1 pathway, and VEGF are key molecular pathways in regulating hair follicle growth and maintenance.

Conclusions: This review found significant aspects exemplified by the properties of probiotics related to promoting hair growth and anti-dandruff effect, which serve as a roadmap for further in-depth studies to make it into pilot scales.

ARTICLE INFO

Keywords: Probiotic Hair loss Alopecia Hair growth Dandruff Scalp

* Corresponding author.

E-mail address: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (T.-H. Yi).

1 These authors contributed equally to this work.

Efficacy and safety of a cream containing panthenol, prebiotics, and probiotic lysate for improving sensitive skin symptoms

Xianghua Zhang1 Delphine Kerob2 Zhongxing Zhang1 Han Tao1 Xiaofeng He3 Yi Yi3 Xiaofeng Fang3 Wenna Wang3 Andrew Steel3

1 L’Oreal Dermatological Beauty, L’Oréal China, Shanghai, China

2 La Roche-Posay Laboratoire Pharmaceutique, Levallois Perret, La Roche-Posay, France

3 Research and Innovation Center, L’Oréal China, Shanghai, China

Correspondence

Xianghua Zhang, L’Oreal Dermatological Beauty, L’Oréal China, Shanghai, China.

Email: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。

Abstract

Background: Sensitive skin is a common condition affecting a significant proportion of the population, and there is a growing demand for effective and safe management.

Aim: To evaluate the efficacy and safety of a cream containing panthenol, prebiotics, and probiotic lysate as an optimal care for facial sensitive skin.

Methods: A total of 110 participants (64 in group A and 46 in group B) with facial sensitive skin applied the cream twice daily for 28 days. Group A evaluated their sensitive skin, product efficacy, and product use experience at D0 (15 min), D1, D14, and D28. In group B, skin barrier function-related indicators were measured at baseline and on D1, D7, D14, and D28. Dermatologists evaluated tolerance for all participants.

Results: After 28 days of use, in group A, 100% of participants reported mildness and comfort with product use. Participants demonstrated significant improvements in skin barrier function-related indicators, including increased stratum corneum moisture content, reduced erythema index, elevated sebum content, decreased trans-epidermal water loss, and diminished skin redness parameter a* value (all p < 0.05). Dermatologist evaluations revealed excellent tolerance among all participants.

Conclusion: The panthenol-enriched cream with prebiotics and probiotic lysate exhibited substantial clinical efficacy in ameliorating facial sensitive skin conditions, coupled with a high safety profile.

KEYWORDS panthenol, prebiotics, probiotics, sensitive skin, skin barrier function