黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
原创:伤口治疗及造口护理中心
足部是糖尿病这个多系统疾病的一个复杂的靶器官。糖尿病患者因周围神经病变与外周血管疾病合并过高的机械压力,可引起足部软组织及骨关节系统的破坏与畸形形成,进而引发一系列足部问题,从轻度的神经症状到严重的溃疡、感染、血管疾病、Charcot关节病和神经病变性骨折。如果积极治疗不能充分解决下肢出现的症状和并发症,则会造成灾难性的后果。因此,在糖尿病患者中开展对足部问题的早期预防和治疗将有重要的意义。
随着现在人们生活水平的逐渐提高,有一种“富贵病”在生活中逐渐出现,渐渐地已经成为生活中的常见病,它就是我们常说的糖尿病。而今天小编不给大家讲糖尿病的相关知识,而是讲一种因糖尿病而引起的一种并发症,它的名字叫糖尿病足!
下肢静脉溃疡
下肢静脉性溃疡,俗称“老烂腿”,是下肢静脉疾病的常见临床表现。下肢静脉性溃疡常常反复发作,溃疡久治不愈,严重影响患者的正常生活,因此一旦出现下肢静脉性溃疡的现象,患者应该及时到正规医院的血管外科就医,以查清病因,对症下药。
糖尿病足骨髓炎(Diabetic foot osteomyelitis,DFO)与更高的踝上截肢率相关。尽管DFO有着很高的发生率和糟糕的预后,然而针对其治疗却缺乏高质量的证据。由于缺乏基于证据的指南,导致其治疗方法多种多样。作者咨询了14个外科专家,采用Delphi方法获得了关于DFO诊断、治疗和管理的专家共识。同时作者希望有更多有力的研究和更清晰的指南,以便能改善外科治疗的结果。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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