黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
原创: 十六点五 中山二院糖尿病足中心
在上节中提到局部鉴别皮肤组织的“死活”是一个难题,有读者问有关在创面表面用“亚甲基蓝”染料染色来鉴别的问题,这种方法是一种比较古老的方法,对于相对较干净的伤口的效果比较好,对于严重感染的伤口容易出现误报,而且为了最大程度的保护足的结构和功能,一般采取蚕食性的方法进行清创,用这些染料染色后,反而有时候会干扰对肉芽组织生长的判断,容易造成清创过多的情况,因此,目前应用较少(也比较难得到有证书能够在临床应用的这种染料)。
针对“杨氏扩散”的特殊性、沿着窦道扩散的缺点及足部手术的原则,我们设计了一种沿着窦道行纵向切开的“刘氏切法”(图1),特点是:(1)沿着窦道做平行于足纵轴切开;(2)一般在窦道开口及窦道末端切开;(3)切口长度3-4CM;(4)足弓部足底边缘部的切口非常关键。
足底溃疡是糖尿病足治疗的难点。尤其是足跟。从理论上讲,足底的组织结构较足背要致密很多,其具有很强的对于损伤的防护及对于细菌感染的抵抗能力,即使在糖尿病状态下,也有一定的防御能力。
在公众号《糖尿病足局部创面的抗菌治疗(2)》中,其实涉及到糖尿病足局部治疗中一个非常重要而关键的问题,就是糖尿病足局部脓肿的切开。但是在《糖尿病足局部创面的抗菌治疗(2)》里面只讲了一些原则,发布之后,有许多读者觉得还是讲的不够透彻,尤其是没有“图解”,很难理解有关的问题,因此,继续补充一些有“图解”的文章,希望能够把有关的问题讲得更加透彻。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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