Tarik Zahra,b,y , Vijay K. Bodac,d,y , Jian Gee,f,y , Lexiang Yua,g , Zhongzhi Wuc,d , Jianwen Quee,f,h, *, Wei Lic,d, *, Li Qianga,g, *
a Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA
b Department of Molecular Pharmacology and Therapeutics, Columbia University, New York, NY 10032, USA
c Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA
d Drug Discovery Center, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA
e Division of Digestive and Liver Diseases, Columbia University, New York, NY 10032, USA
f Center for Human Development, Columbia University, New York, NY 10027, USA
g Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
h Department of Medicine, Columbia University, New York, NY 10032, USA
*Corresponding authors.
E-mail addresses: jq2240@cumc.columbia.edu (Jianwen Que), wli@uthsc.edu (Wei Li), lq2123@cumc.columbia.edu (Li Qiang).
These authors made equal contributions to this work.
Peer review under the responsibility of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences.
Received 22 October 2023; received in revised form 13 December 2023; accepted 5 January 2024
KEY WORDS Estrogen receptor b; Aging; Metabolism; Skin injury; Muscle metabolism; Small molecule conjugates; Regeneration; Adiposity
Hanan Khojah a , Shaima R. Ahmed a,* , Shahad Y. Alharbi a , Kholood K. AlSabeelah a ,
Hatham Y. Alrayyes a , Kadi B. Almusayyab a , Shahad R. Alrawiliy a , Raghad M. Alshammari a ,
Sumera Qasim b
a Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia
b Department of Pharmacology, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia
Corresponding author at: College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia.
E-mail address: Srmorsi@ju.edu.sa (S.R. Ahmed)
ARTICLE INFO
Keywords:
Launaea procumbens
Anti-aging
ADMET
Docking
ABSTRACT
Aging is a natural process that occurs in all living organisms. Particularly, the skin embodies aging since it serves as a barrier between the body and its surroundings. Previously, we reported the wound healing effect of Launaea procumbens and identified compounds therein. The study aims to explore the skin anti-aging properties of the plant extract. To that effect, the antioxidant potential of L. procumbens methanolic extract (LPM) was assessed using two complementary DPPH and FRAP assays. The enzyme inhibitory effect of the extract on collagenase, elastase, hyaluronidase, and tyrosinase was evaluated to assess the direct skin anti-aging effects. Similarly, the anti-inflammatory activity was evaluated to explore the indirect anti-aging effects via the assessment of extract inhibitory effects on cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). In addition, ADMET and molecular docking studies were performed to explore the interaction mechanisms of identified compounds in LPM with target enzymes. LPM demonstrated significant antioxidant activity in DPPH (IC50 = 29.08 µg/mL) and FRAP (1214.67 µM FeSO4/g extract) assays. Plant extract showed significant inhibition of collagenase, elastase, hyaluronidase, and tyrosinase (IC50 = 52.68, 43.76, 31.031, and 37.13 µg/mL, respectively). The extract demonstrated significant COX-2 and 5-LOX inhibition capacity with IC50 values of 8.635 and 10.851 µg/mL, respectively. The molecular docking study revealed the high potential of the identified compounds to bind to the active sites of enzymes crucially involved in the skin aging process. ADMET analysis of the compounds revealed their good absorption, distribution, and metabolism profiles, and they were found to be safe as well. Study findings suggest L. procumbens as a promising source for the development of natural skin anti-aging and anti oxidant compounds. This, in turn, may facilitate its incorporation into cosmetic formulations after further investigation.
原创: 十六点五 中山二院糖尿病足中心
男性,24岁,3年前外院诊断“下丘脑生殖细胞瘤”,进行γ刀治疗后,长期应用“氢化可的松”早晚各2片维持,半年前不慎损伤左小腿下部外侧皮肤,之后长期不愈合,收入院。
含银的敷料是目前治疗糖尿病足的主流敷料,有各种剂型,包括泡沫状、胶冻态、液态、粉末态、膏态等等,生产的厂家的非常多,其中的质量良莠不齐,很难鉴别。
进一步的清创就到了骨骼,对于骨髓炎最彻底的治疗方法就是去除被细菌侵袭的骨,正常的骨骼一般是浅黄色,如果发白或者变黑,往往就提示这段骨头可能活性比较差,另外,还有一种是面积比较大的骨暴露,一般的肉芽组织很难爬上去,因此,对于坏死的骨头及较大面积的骨暴露,一般需要清除。
皮下组织中的脂肪组织在足部分布广泛,而皮下脂肪中血管分布非常少,在相对或绝对缺血的糖尿病足中,脂肪组织边缘的是否出血不是其是否存活的可靠指标。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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