Surface Inactivation of a SARS‑CoV‑2 Surrogate with Hypochlorous Acid is Impacted by Surface Type, Contact Time, Inoculum Matrix, and Concentration

Allyson N. Hamilton¹  · Sahaana Chandran¹  · Christopher A. Baker^1,2 · Kristen E. Gibson¹

Received: 2 December 2022 / Accepted: 13 January 2023 / Published online: 21 January 2023

© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023

Abstract

Indirect contact with contaminated surfaces is a potential transmission route for COVID-19. Therefore, it is necessary to investigate convenient and inexpensive surface sanitization methods, such as HOCl, against severe acute respiratory syn-drome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 surrogate, Phi6 (~7 log PFU/mL), was prepared in artificial saliva and tripartite matrices, spot inoculated on coupons of either stainless steel or vinyl, and allowed to dry. The coupons were sprayed with either 500 ppm or 1000 ppm HOCl, and remained on the surface for 0 s (control), 5 s, 30 s, or 60 s. Samples were enumerated via the double agar overlay assay. Statistical analysis was completed in R using a generalized linear model with Quasipoisson error approximations. Time, concentration, surface type, and inoculum matrix were all significant contributors to log reduction at P=0.05. Significant three-way interactions were observed for 1000 ppm, vinyl, and 60 s (P=0.03) and 1000 ppm, tripartite, and 60 s (P=0.0121). A significant two-way interaction between vinyl and 60 s was also observed (P=0.0168). Overall, increased HOCl concentration and exposure time led to increased Phi6 reduction. Notably, the highest estimated mean log reduction was 3.31 (95% CI 3.14, 3.49) for stainless steel at 60 s and 1000 ppm HOCl in artificial saliva, indicating that this method of sanitization may not adequately reduce enveloped viruses to below infective thresholds.

Keywords Fomite · Phi6 · Hypochlorous acid · Vinyl · Stainless steel

Correlation and Agreement Between Transcutaneous Oxygen Pressure and Toe Pressure in Patients with Diabetic Foot Ulcers

Kyu-Il Lee¹ , Yu-Kyeong Yun² , Seung-Kyu Han^2,3 , Kyung-Chul Moon^2,3 , Sik Namgoong^2,3 ,

Seong-Ho Jeong³ , Eun-Sang Dhong³

¹Department of Plastic Surgery, Taean Public Health Office, Taean, Korea

²Diabetic Wound Center and Department of Plastic Surgery, Korea University Guro Hospital, Seoul, Korea

³Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea

Abstract

Background: Adequate tissue oxygenation is a key determinant of diabetic foot ulcer (DFU) outcomes. Though transcutaneous oxygen pressure (TcPO₂) is the gold standard for evaluating tissue oxygenation, its limited availability restricts routine clinical use. Consequently, toe pressure is frequently utilized as a practical surrogate; however, the direct correlation between these two modalities has yet to be rigorously investigated. This study aimed to assess the correlation and agreement between TcPO₂and toe pressure in patients with DFUs.

Methods: A retrospective review was conducted on 837 DFU patients who received simultaneous TcPO²and toe pressure assessments.

The correlation between the two tests was analyzed using the Pearson correlation coefficient, and agreement was evaluated using Bland– Altman analysis. To aid interpretation, a scatterplot and Bland–Altman plot were generated.

Results: TcPO₂and toe pressure demonstrated a strong correlation (R=0.66; 95% confidence interval, 0.62 to 0.70; P<0.001). Bland–

Altman analysis showed a mean bias of 26.9 mmHg (standard deviation of differences, 28.8 mmHg; 95% limits of agreement, −28.9 to 82.6 mmHg) between toe pressure and TcPO₂, reflecting limited agreement and increased variability at higher perfusion levels.

Conclusion: TcPO₂and toe pressure are strongly correlated. However, they are not interchangeable, particularly in DFU patients with high tissue perfusion.

Keywords: Diabetic foot; Foot ulcer; Ischemia; Microcirculation

Status Report on Topical Hypochlorous Acid: Clinical Relevance of Specific Formulations, Potential Modes of Action, and Study Outcomes

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Effect of Stabilized Hypochlorous Acid on Re-epithelialization and Bacterial Bioburden in Acute Wounds: A Randomized Controlled Trial in Healthy Volunteers

Ewa A. BURIAN¹, Lubna SABAH¹, Klaus KIRKETERP-MØLLER¹, Glenn GUNDERSEN² and Magnus S. ÅGREN^1,3,4

¹Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark,
²SoftOx Solutions AS, Oslo, Norway, ³Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, and ⁴Department of Clinical
Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting

Yuta Tsujisaka,^1,2,5 Takeshi Hatani,^1,2,5 Chikako Okubo,¹ Ryo Ito,¹ Azuma Kimura,¹ Megumi Narita,¹

Kazuhisa Chonabayashi,^1,3 Shunsuke Funakoshi,^1,4 Antonio Lucena-Cacace,¹ Taro Toyoda,1 Kenji Osafune,¹

Takeshi Kimura,² Hirohide Saito,^{1, *} and Yoshinori Yoshida^{1,4, *}

¹Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan

²Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan

³Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan

⁴Takeda-CiRA Joint Program (T-CiRA), Fujisawa 251-0012, Japan

⁵These authors contributed equally

*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.S.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Y.Y.)

https://doi.org/10.1016/j.stemcr.2022.05.003

SUMMARY
For regenerative cell therapies using pluripotent stem cell (PSC)-derived cells, large quantities of purified cells are required. Magnetic-activated cell sorting (MACS) is a powerful approach to collect target antigen-positive cells; however, it remains a challenge to purify various cell types efficiently at large scale without using antibodies specific to the desired cell type. Here we develop a technology that combines microRNA (miRNA)-responsive mRNA switch (miR-switch) with MACS (miR-switch-MACS) to purify large amounts of PSC-derived cells rapidly and effectively. We designed miR-switches that detect specific miRNAs expressed in target cells and controlled the translation of a CD4-coding transgene as a selection marker for MACS. For the large-scale purification of induced PSC-derived cardiomyocytes (iPSC-CMs), we transferred miR-208a-CD4 switch-MACS and obtained purified iPSC-CMs efficiently. Moreover, miR-375-CD4 switch-MACS highly purified pancreatic insulin-producing cells and their progenitors expressing Chromogranin A. Overall, the miR-switch-MACS method can efficiently purify target PSC-derived cells for cell replacement therapy.

局部冷敷处理在眼部整形美容术后的应用

白 玲

兰州普瑞眼科医院 甘肃兰州

【摘要】目的 探寻在眼部整形美容术后实行局部冷敷处理的效果。方法 选择我院收治的 60 例眼部整形美容术患者，随机平分成对照组和观察组，分别实行术后常规处理和局部冷敷处理，对比效果。结果 观察组创口开始好转时间（2.11±1.24）、创口基本愈合时间（5.67±2.04）、术眼局部肿胀消退时间（2.04±0.71）明显优于对照组（P＜0.05）；观察组术后创口疼痛程度明显低于对照组（P＜0.05）；观察组 SAS 和 SDS 评分明显低于对照组（P＜0.05），观察组睡眠质量明显高于对照组（P＜0.05）；观察组术后不良反应明显低于对照组（P＜0.05）。结论 局部冷敷处理可使眼部整形美容术后患者的临床各项指标改善，减少疼痛状况，缓解负面情绪，使睡眠质量提升，使不良反应发生率降低。

【关键词】术后；眼部整形美容；局部冷敷处理

【收稿日期】2024 年 2 月 17 日 【出刊日期】2024 年 3 月 25 日 【DOI】10.12208/j.ijcr.20240101

Application of local cold compress treatment in eye plastic surgery

Ling Bai

Lanzhou Purui Eye Hospital, Lanzhou, Gansu

【Abstract】Objective To explore the effect of local cold compress after eye cosmetic surgery. Methods 60 patients in our hospital were randomly divided into control group and observation group, and the postoperative routine treatment and local cold application were applied respectively to compare the effect. Results The observation group (2.11 ± 1.24), basic wound healing time (5.67 ± 2.04), and local swelling regression time (2.04 ± 0.71) were significantly better than controls (P <0.05); significantly lower (P <0.05); SAS and SDS scores were significantly lower (P <0.05) (P <0.05); the observation group was significantly lower than the control group (P <0.05). Conclusion Local cold compress treatment can improve the clinical indicators of patients after eye plastic surgery, reduce the pain status, relieve the negative emotions, improve the sleep quality, and reduce the incidence of adverse reactions.

【Keywords】Postoperative; Eye plastic surgery; Local cold compress treatment