
伤口世界

- 星期五, 11 7月 2025
Resurrection of endogenous retroviruses during aging reinforces senescence
Xiaoqian Liu,1,5,6,7,21 Zunpeng Liu,1,5,7,21 Zeming Wu,2,5,7,21 Jie Ren,3,5,7,21 Yanling Fan,3,7 Liang Sun,9 Gang Cao,8 Yuyu Niu,11,12,13 Baohu Zhang,1,7 Qianzhao Ji,2,7 Xiaoyu Jiang,2,7 Cui Wang,3,7 Qiaoran Wang,3,7 Zhejun Ji,1,5,7 Lanzhu Li,2,7 Concepcion Rodriguez Esteban,18 Kaowen Yan,2,5,7 Wei Li,4 Yusheng Cai,2,5,7 Si Wang,4,5,7,15,16 Aihua Zheng,7,19 Yong E. Zhang,7,14 Shengjun Tan,14 Yingao Cai,7,14 Moshi Song,2,5,6,7 Falong Lu,7,10 Fuchou Tang,17 Weizhi Ji,11,12,20 Qi Zhou,1,5,6,7,20 Juan Carlos Izpisua Belmonte,18,20 Weiqi Zhang,3,5,7,* Jing Qu,1,5,6,7,* and Guang-Hui Liu2,4,5,6,7,22,*
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2 State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
3 CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing 100101, China
4 Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China
5 Institute for Stem cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China
6 Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China
7 University of Chinese Academy of Sciences, Beijing 100049, China
8 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
9 NHC Beijing Institute of Geriatrics, NHC Key Laboratory of Geriatrics, Institute of Geriatric Medicine of Chinese Academy of Medical Sciences, National Center of Gerontology/Beijing Hospital, Beijing 100730, China
10 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
11 State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China
12 Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan 650500, China
13 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China
14 Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
15 Aging Translational Medicine Center, International Center for Aging and Cancer, Xuanwu Hospital, Capital Medical University, Beijing Municipal Geriatric Medical Research Center, Beijing 100053, China
16 The Fifth People’s Hospital of Chongqing, Chongqing 400062, China
17 Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
18 Altos Labs, Inc., San Diego, CA 94022, USA
19 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
20 Senior author
21 These authors contributed equally
22 Lead contact
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (W.Z.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.Q.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (G.-H.L.)
https://doi.org/10.1016/j.cell.2022.12.017
SUMMARY
Whether and how certain transposable elements with viral origins, such as endogenous retroviruses (ERVs) dormant in our genomes, can become awakened and contribute to the aging process is largely unknown. In human senescent cells, we found that HERVK (HML-2), the most recently integrated human ERVs, are unlocked to transcribe viral genes and produce retrovirus-like particles (RVLPs). These HERVK RVLPs constitute a transmissible message to elicit senescence phenotypes in young cells, which can be blocked by neutralizing antibodies. The activation of ERVs was also observed in organs of aged primates and mice as well as in human tissues and serum from the elderly. Their repression alleviates cellular senescence and tissue degeneration and, to some extent, organismal aging. These findings indicate that the resurrection of ERVs is a hallmark and driving force of cellular senescence and tissue aging.
- 星期五, 04 7月 2025
Motor and vestibular signals in the visual cortex permit the separation of self versus externally generated visual motion
Mateo Ve´ lez-Fort,1,3 Lee Cossell,1,3 Laura Porta,1 Claudia Clopath,1,2 and Troy W. Margrie1,4,*
1 Sainsbury Wellcome Centre, University College London, London, UK
2 Bioengineering Department, Imperial College London, London, UK
3 These authors contributed equally
4 Lead contact
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https://doi.org/10.1016/j.cell.2025.01.032
SUMMARY
Knowing whether we are moving or something in the world is moving around us is possibly the most critical sensory discrimination we need to perform. How the brain and, in particular, the visual system solves this motion-source separation problem is not known. Here, we find that motor, vestibular, and visual motion signals are used by the mouse primary visual cortex (VISp) to differentially represent the same visual flow information according to whether the head is stationary or experiencing passive versus active translation. During locomotion, we find that running suppresses running-congruent translation input and that translation signals dominate VISp activity when running and translation speed become incongruent. This cross-modal interaction between the motor and vestibular systems was found throughout the cortex, indicating that running and translation signals provide a brain-wide egocentric reference frame for computing the internally generated and actual speed of self when moving through and sensing the external world.

- 星期四, 03 7月 2025
Mapping the landscape of social behavior
Ugne Klibaite,1,4,* Tianqing Li,2,4 Diego Aldarondo,1,3 Jumana F. Akoad,1 Bence P. O¨ lveczky,1,* and Timothy W. Dunn2,5,*
1 Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
2 Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
3 Present address: Fauna Robotics, New York, NY 10003, USA
4 These authors contributed equally
5 Lead contact
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (U.K.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (B.P.O¨ .), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (T.W.D.)
https://doi.org/10.1016/j.cell.2025.01.044
SUMMARY
Social interaction is integral to animal behavior. However, lacking tools to describe it in quantitative and rigorous ways has limited our understanding of its structure, underlying principles, and the neuropsychiatric disorders, like autism, that perturb it. Here, we present a technique for high-resolution 3D tracking of postural dynamics and social touch in freely interacting animals, solving the challenging subject occlusion and partassignment problems using 3D geometric reasoning, graph neural networks, and semi-supervised learning. We collected over 110 million 3D pose samples in interacting rats and mice, including seven monogenic autism rat lines. Using a multi-scale embedding approach, we identified a rich landscape of stereotyped actions, interactions, synchrony, and body contacts. This high-resolution phenotyping revealed a spectrum of changes in autism models and in response to amphetamine not resolved by conventional measurements. Our framework and large library of interactions will facilitate studies of social behaviors and their neurobiological underpinnings.

- 星期四, 26 6月 2025
Large language models deconstruct the clinical intuition behind diagnosing autism
Jack Stanley,1,2,6 Emmett Rabot,3,4,6 Siva Reddy,1 Eugene Belilovsky,1,5 Laurent Mottron,3,4,7 and Danilo Bzdok1,2,7,8,*
1 Mila - Que´ bec Artificial Intelligence Institute, Montre´ al, QC H2S3H1, Canada
2 The Neuro - Montre´ al Neurological Institute (MNI), McConnell Brain Imaging Centre, Department of Biomedical Engineering, Faculty of Medicine, School of Computer Science, McGill University, Montre´ al, QC H3A2B4, Canada
3 Research Center, Centre Inte´ gre´ Universitaire de Sante´ et de Services Sociaux du Nord-de-lIle-de-Montre´ al (CIUSSS-NIM), Montre´ al, QC H4K1B3, Canada
4 Universite´ de Montre´ al, Montre´ al, QC H3C3J7, Canada
5 Department of Computer Science and Software Engineering, Concordia University, Montreal, QC H3G 1M8, Canada
6 These authors contributed equally
7 These authors contributed equally
8 Lead contact
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https://doi.org/10.1016/j.cell.2025.02.025
SUMMARY
Efforts to use genome-wide assays or brain scans to diagnose autism have seen diminishing returns. Yet the clinical intuition of healthcare professionals, based on longstanding first-hand experience, remains the gold standard for diagnosis of autism. We leveraged deep learning to deconstruct and interrogate the logic of expert clinician intuition from clinical reports to inform our understanding of autism. After pre-training on hundreds of millions of general sentences, we finessed large language models (LLMs) on >4,000 free-form health records from healthcare professionals to distinguish confirmed versus suspected autism cases. By introducing an explainability strategy, our extended language model architecture could pin down the most salient single sentences in what drives clinical thinking toward correct diagnoses. Our framework flagged the most autism-critical DSM-5 criteria to be stereotyped repetitive behaviors, special interests, and perception-based behaviors, which challenges today,s focus on deficits in social interplay, suggesting necessary revision of long-trusted diagnostic criteria in gold-standard instruments.

- 星期二, 24 6月 2025
Jumbo phage killer immune system targets early infection of nucleus-forming phages
Li Yuping,1,7,* Linlin Guan,2 Isabelle Becher,3 Kira S. Makarova,4 Xueli Cao,2 Surabhi Hareendranath,1 Jingwen Guan,1 Frank Stein,3 Siqi Yang,2 Arne Boergel,3 Karine Lapouge,3 Kim Remans,3 David Agard,5 Mikhail Savitski,3 Athanasios Typas,3 Eugene V. Koonin,4 Yue Feng,2,* and Joseph Bondy-Denomy1,6,8,*
1 Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94403, USA
2 State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
3 European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, Germany
4 Computational Biology Branch, Division of Intramural Research, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
5 The Chan-Zuckerberg Institute for Advanced Biological Imaging and the Department of Biochemistry, University of California, San Francisco, San Francisco, CA 94143, USA
6 Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94403, USA
7 Present address: Biozentrum, University of Basel, Basel 4056, Switzerland
8 Lead contact
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (L.Y.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Y.F.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.B.-D.)
https://doi.org/10.1016/j.cell.2025.02.016
SUMMARY
Jumbo bacteriophages of the fKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify ‘‘jumbo phage killer’’ (Juk), a two-component immune system that terminates infection of fKZ-like phages, suppressing the expression of early phage genes and preventing phage DNA replication and phage nucleus assembly while saving the cell. JukA (formerly YaaW) rapidly senses the EPI vesicle by binding to an early-expressed phage protein, gp241, and then directly recruits JukB. The JukB effector structurally resembles a pore-forming toxin and destabilizes the EPI vesicle. Functional anti-fKZ JukA homologs are found across bacterial phyla, associated with diverse effectors. These findings reveal a widespread defense system that specifically targets early events executed by fKZ-like jumbo phages prior to phage nucleus assembly.