Ha Young Park a , Min Ho Kang a , Guewha Lee b , Jin Woo Kim a,c,*
a Department of Food Science, Sunmoon University, Chungcheongnam-do, Republic of Korea
b Hu evergreen Pharm Inc., Incheon, Republic of Korea
c Center for Next-Generation Semiconductor Technology, Sun Moon University, Chungnam, Republic of Korea
ARTICLE INFO
Keywords: Ginseng non-edible callus Extracellular vesicle Skin regeneration Collagen synthesis Proliferation
1 . ABSTRACT
Background: This study aimed to investigate the effects of ginseng non-edible callus-derived extracellular vesicle (GNEV) on skin regeneration, particularly focusing on its impact on proliferation and migration in human dermal fibroblast (HDF).
Methods: GNEV was isolated from ginseng non-edible callus using sequential filtration and size exclusion chromatography (SEC). The extracellular vesicle was characterized using nanoparticle tracking analysis (NTA). HDF was treated with various concentrations of GNEV, and cell viability, proliferation, and migration were assessed using MTT and scratch wound healing assays. Gene expression related to collagen synthesis (TGF-β, SMAD-2, SMAD-3, COL1A1) was measured using RT-PCR.
Results: Treatment of HDF with GNEV resulted in a significant 2.5-fold increase in cell migration compared to the non-treated group. Furthermore, GNEV demonstrated the upregulation of collagen synthesis genes, specifically TGF-β, SMAD-2, SMAD-3, and COL1A1, by 41.7 %, 59.4 %, 60.2 %, and 21.8 %, respectively. These findings indicated that GNEV activates the TGF-β/SMAD signaling pathway, showcasing its potential to induce skin
Conclusions: In conclusion, GNEV exhibits a notable ability to enhance skin regeneration through its stimulatory effects on cell migration and the upregulation of key collagen synthesis genes. The activation of the TGF-β/SMAD signaling pathway further suggests the potential of GNEV as a promising candidate for drug delivery systems in the fields of cosmetics and pharmaceuticals, opening avenues for further research and application in skincare and dermatology
* Corresponding author. Department of Food Science, Sunmoon University 70, Sunmoon-ro 221 beon-gil, Tangjeong-myeon, Asan-si, Chungcheongnam-do, Re
public of Korea.
E-mail addresses: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.Y. Park), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (M.H. Kang), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (G. Lee), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
(J.W. Kim).
Contents lists available at ScienceDirect
Journal of Ginseng Research
journal homepage: www.sciencedirect.com/journal/journal-of-ginseng-research
https://doi.org/10.1016/j.jgr.2024.08.002
Received 22 February 2024; Received in revised form 7 August 2024; Accepted 16 August 2024
Journal of Ginseng Research 49 (2025) 34–41
Available online 23 August 2024
1226-8453/© 2024 The Korean Society of Ginseng. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
This article is excerpted from the Journal of Ginseng Research 49 (2025) 34–41 by Wound World.
