Marta Arnal‑Forné1 · Tamara Molina‑García2 · María Ortega2 · Víctor Marcos‑Garcés2,3 · Pilar Molina4 · Antonio Ferrández‑Izquierdo1,2,5 · Pilar Sepulveda1,6,7 · Vicente Bodí2,3,6,8 · César Ríos‑Navarro1,2,6 · Amparo Ruiz‑Saurí1,2,6
Accepted: 10 June 2024 / Published online: 2 July 2024
© The Author(s) 2024
Abstract
Skin represents the main barrier against the external environment, but also plays a role in human relations, as one of the prime determinants of beauty, resulting in a high consumer demand for skincare-related pharmaceutical products. Given the importance of skin aging in both medical and social spheres, the present research aims to characterize microscopic changes in human skin composition due to intrinsic aging (as opposed to aging infuenced by external factors) via histological analysis of a photoprotected body region. Samples from 25 autopsies were taken from the periumbilical area and classifed into four age groups: group 1 (0–12 years), group 2 (13–25 years), group 3 (26–54 years), and group 4 (≥55 years). Diferent traditional histological (hematoxylin–eosin, Masson’s trichrome, orcein, toluidine, Alcian blue, and Feulgen reaction) and immunohistochemical (CK20, CD1a, Ki67, and CD31) stains were performed. A total of 1879 images photographed with a Leica DM3000 optical microscope were morphometrically analyzed using Image ProPlus 7.0 for further statistical analysis with GraphPad 9.0. Our results showed a reduction in epidermis thickness, interdigitation and mitotic indexes, while melanocyte count was raised. Papillary but not reticular dermis showed increased thickness with aging. Specifcally, in the papillary layer mast cells and glycosaminoglycans were expanded, whereas the reticular dermis displayed a diminution in glycosaminoglycans and elastic fbers. Moreover, total cellularity and vascularization of both dermises were diminished with aging. This morphometric analysis of photoprotected areas reveals that intrinsic aging signifcantly infuences human skin composition. This study paves the way for further research into the molecular basis underpinning these alterations, and into potential antiaging strategies.
Keywords Skin aging · Intrinsic aging · Morphometric analysis · Human biopsies
César Ríos-Navarro
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1 Department of Pathology, University of Valencia, Avda. Blasco Ibáñez 15. 46010, Valencia, Spain
2 Instituto de Investigación Sanitaria INCLIVA Biomedical Research Institute, Avda. Menéndez Pelayo 4acc,
46010 Valencia, Spain
3 Cardiology Department, Hospital Clínico Universitario, Valencia, Spain
4 Department of Pathology, Instituto de Medicina Legal y Ciencias Forenses, Valencia, Spain
5 Anatomic Pathology Department, Hospital Clínico Universitario, Valencia, Spain
6 Centro de Investigación Biomédica en Red (CIBER)-CV, Madrid, Spain
7 Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain
8 Department of Medicine, University of Valencia, Valencia, Spain
Nguyen Ngan Giang1,2 Hyun Ji Kim3 Pham Ngoc Chien2,3 Han Jin Kwon4 Jung Ryul Ham4 Won Ku Lee4 Yeon Ju Gu4 Shou Yi Zhou2,5 Xin Rui Zhang2,5 Sun Young Nam2 Chan Yeong Heo1,2,3,5
1 Department of Medical Device Development, College of Medicine, Seoul National University, Seoul, Republic of Korea
2 Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam, South Korea
3 Korea Institute of Nonclinical Study, H&Bio. co. Ltd., Seongnam, Republic of Korea
4 UltraV Co., Ltd. R&D Center, Seoul, South Korea
5 Department of Medicine, College of Medicine, Seoul National University, Seoul, Republic of Korea
Correspondence Sun Young Nam and Chan Yeong Heo, Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam, Korea.
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Background: Injectable filler, a nonsurgical beauty method, has gained popularity in rejuvenating sagging skin. In this study, polydioxanone (PDO) was utilized as the main component of the ULTRACOL200 filler that helps stimulate collagenesis and provide skin radiant effects. The study aimed to evaluate and compare the effectiveness of ULTRACOL200 with other commercialized products in visually improving dermatological problems.
Methods: Herein, 31 participants aged between 20 and 59 years were enrolled in the study. 1 mL of the testing product, as well as the quantity for the compared groups was injected into each participants face side individually. Subsequently, skin texture and sunken volume of skin were measured using ANTERA 3D CS imaging technology at three periods: before the application, 4 weeks after the initial application, and 4 weeks after the 2nd application of ULTRACOL200.
Results: The final results of skin texture and wrinkle volume evaluation consistently demonstrated significant enhancement. Consequently, subjective questionnaires were provided to the participants to evaluate the efficacy of the testing product, illustrating satisfactory responses after the twice applications.
Conclusion: The investigation has contributed substantially to the comprehension of a PDO-based filler (ULTRACOL200) for skin enhancement and provided profound insight for future clinical trials.
KEYWORDS collagen formation, filler, polydioxanone, skin enhancement, Ultracol200
Dr路遥 骨科时间
外科手术，几乎都会有缝（钉）合的伤口。手术后，患者最担心的也就是伤口感染。
实际上，外科医生比患者更害怕这个，所以他们才会穷尽所能的在各方面严格要求，以期避免切口感染。
伤口处理的基本原则
崔飞飞 东阳市人民医院
引言 — 伤口是皮肤及皮肤系统正常结构和功能的破坏[1]。急性伤口有正常的伤口生理机能，预期其愈合可以通过正常的伤口愈合过程，而慢性伤口则被定义为生理机能受损[2,3]。
Authors
Deniz Harputlu
Süheyla A. Özsoy
Maarit Ahtiala
Esa Soppi
Teijo I. Saari
Keywords
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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