伤口世界

电子邮件地址: michael.kung@139.com

星期四, 07 11月 2019

EMPIRICAL STUDIES Cultivating Incontinence-associated Dermatitis Prevention Practices in an Australian Local Health District: A Quasi-experimental Study

Authors

Michelle Barakat-Johnson

星期四, 07 11月 2019

INDUSTRY SPOTLIGHT Industry Spotlight: Graham Dixon, BSc, PhD, CEO and Executive Director, Neem Biotech

Issue: Volume 64 - Issue 12 - December 2018 ISSN 1943-2720

Index: Ostomy Wound Manage. 2018;64(12).

星期四, 07 11月 2019

SPECIAL REPORT Working with Persons Who Inject Drugs: Be Aware of Wound Botulism

Authors

Barbara A. Pieper

星期四, 07 11月 2019

SPECIAL REPORT Wound Care Stakeholders Advocacy Results in Positive Changes in Medicare Coding Process

Authors

Marcia Nusgart

星期四, 07 11月 2019

EMPIRICAL STUDIES A Single-arm, Practical Application Assessment of User Experience and Peristomal Skin Condition Among Persons With an Ileostomy Using a New Barrier Seal With Assisted Flow

Authors

Kevin Kelleher

Rhona Hunt

星期四, 07 11月 2019

EMPIRICAL STUDIES Development of a New Incontinence Containment Product and an Investigation of Its Effect on Perineal Dermatitis in Patients With Fecal Incontinence: A Pilot Study in Women

Authors

Hülya Leblebicioğlu

Leyla Khorshid

K2 Content

Large language models deconstruct the clinical intuition behind diagnosing autism 2025-06-26 00:00

Jack Stanley,1,2,6 Emmett Rabot,3,4,6 Siva Reddy,1 Eugene Belilovsky,1,5 Laurent Mottron,3,4,7 and Danilo Bzdok1,2,7,8,*

1 Mila - Que´ bec Artificial Intelligence Institute, Montre´ al, QC H2S3H1, Canada

2 The Neuro - Montre´ al Neurological Institute (MNI), McConnell Brain Imaging Centre, Department of Biomedical Engineering, Faculty of Medicine, School of Computer Science, McGill University, Montre´ al, QC H3A2B4, Canada

3 Research Center, Centre Inte´ gre´ Universitaire de Sante´ et de Services Sociaux du Nord-de-lIle-de-Montre´ al (CIUSSS-NIM), Montre´ al, QC H4K1B3, Canada

4 Universite´ de Montre´ al, Montre´ al, QC H3C3J7, Canada

5 Department of Computer Science and Software Engineering, Concordia University, Montreal, QC H3G 1M8, Canada

6 These authors contributed equally

7 These authors contributed equally

8 Lead contact

*Correspondence: danilo.bzdok@mcgill.ca

https://doi.org/10.1016/j.cell.2025.02.025

SUMMARY

Efforts to use genome-wide assays or brain scans to diagnose autism have seen diminishing returns. Yet the clinical intuition of healthcare professionals, based on longstanding first-hand experience, remains the gold standard for diagnosis of autism. We leveraged deep learning to deconstruct and interrogate the logic of expert clinician intuition from clinical reports to inform our understanding of autism. After pre-training on hundreds of millions of general sentences, we finessed large language models (LLMs) on >4,000 free-form health records from healthcare professionals to distinguish confirmed versus suspected autism cases. By introducing an explainability strategy, our extended language model architecture could pin down the most salient single sentences in what drives clinical thinking toward correct diagnoses. Our framework flagged the most autism-critical DSM-5 criteria to be stereotyped repetitive behaviors, special interests, and perception-based behaviors, which challenges today,s focus on deficits in social interplay, suggesting necessary revision of long-trusted diagnostic criteria in gold-standard instruments.
Jumbo phage killer immune system targets early infection of nucleus-forming phages 2025-06-24 00:00

Li Yuping,1,7,* Linlin Guan,2 Isabelle Becher,3 Kira S. Makarova,4 Xueli Cao,2 Surabhi Hareendranath,1 Jingwen Guan,1 Frank Stein,3 Siqi Yang,2 Arne Boergel,3 Karine Lapouge,3 Kim Remans,3 David Agard,5 Mikhail Savitski,3 Athanasios Typas,3 Eugene V. Koonin,4 Yue Feng,2,* and Joseph Bondy-Denomy1,6,8,*

1 Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94403, USA

2 State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

3 European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, Germany

4 Computational Biology Branch, Division of Intramural Research, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA

5 The Chan-Zuckerberg Institute for Advanced Biological Imaging and the Department of Biochemistry, University of California, San Francisco, San Francisco, CA 94143, USA

6 Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94403, USA

7 Present address: Biozentrum, University of Basel, Basel 4056, Switzerland

8 Lead contact

*Correspondence: yuping.li@unibas.ch (L.Y.), fengyue@mail.buct.edu.cn (Y.F.), joseph.bondy-denomy@ucsf.edu (J.B.-D.)

https://doi.org/10.1016/j.cell.2025.02.016

SUMMARY

Jumbo bacteriophages of the fKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify ‘‘jumbo phage killer’’ (Juk), a two-component immune system that terminates infection of fKZ-like phages, suppressing the expression of early phage genes and preventing phage DNA replication and phage nucleus assembly while saving the cell. JukA (formerly YaaW) rapidly senses the EPI vesicle by binding to an early-expressed phage protein, gp241, and then directly recruits JukB. The JukB effector structurally resembles a pore-forming toxin and destabilizes the EPI vesicle. Functional anti-fKZ JukA homologs are found across bacterial phyla, associated with diverse effectors. These findings reveal a widespread defense system that specifically targets early events executed by fKZ-like jumbo phages prior to phage nucleus assembly.
n-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-ET 2025-06-23 00:00

Sven Klumpe,1,9,* Kirsten A. Senti,2 Florian Beck,1 Jenny Sachweh,3 Bernhard Hampoelz,3 Paolo Ronchi,4 Viola Oorschot,4 Marlene Brandstetter,6 Assa Yeroslaviz,5 John A.G. Briggs,7 Julius Brennecke,2,* Martin Beck,3,8,* and Ju¨ rgen M. Plitzko1,*

1 Research Group CryoEM Technology, Max Planck Institute of Biochemistry, Martinsried, Germany

2 Institute of Molecular Biotechnology Austria (IMBA), Vienna, Austria

3 Department Molecular Sociology, Max Planck Institute of Biophysics, Frankfurt, Germany

4 EMBL EM Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany

5 Computational Systems Biochemistry, Bioinformatics Core Facility, Max Planck Institute of Biochemistry, Martinsried, Germany

6 Electron Microscopy Facility, Vienna BioCenter Core Facilities, Vienna, Austria

7 Department of Cell and Virus Structure, Max Planck Institute of Biochemistry, Martinsried, Germany

8 Institute of Biochemistry, Goethe University Frankfurt, Frankfurt, Germany

9 Lead contact

*Correspondence: klumpe@biochem.mpg.de (S.K.), julius.brennecke@imba.oaew.ac.at (J.B.), martin.beck@biophys.mpg.de (M.B.), plitzko@biochem.mpg.de (J.M.P.)

https://doi.org/10.1016/j.cell.2025.02.003

SUMMARY

Long terminal repeat (LTR) retrotransposons belong to the transposable elements (TEs), autonomously replicating genetic elements that integrate into the host,s genome. Among animals, Drosophila melanogaster serves as an important model organism for TE research and contains several LTR retrotransposons, including the Ty1-copia family, which is evolutionarily related to retroviruses and forms viruslike particles (VLPs). In this study, we use cryo-focused ion beam (FIB) milling and lift-out approaches to visualize copia VLPs in ovarian cells and intact egg chambers, resolving the in situ copia capsid structure to 7.7 A˚ resolution by cryoelectron tomography (cryo-ET). Although cytoplasmic copia VLPs vary in size, nuclear VLPs are homogeneous and form densely packed clusters, supporting a model in which nuclear import acts as a size selector. Analyzing flies deficient in the TE-suppressing PIWI-interacting RNA (piRNA) pathway, we observe copia,s translocation into the nucleus during spermatogenesis. Our findings provide insights into the replication cycle and cellular structural biology of an active LTR retrotransposon.

Custom Mod Mega1

主任医师、教授、博导，南方医科大学第三附属医院（广东省骨科医院）院长

伤口世界

EMPIRICAL STUDIES Cultivating Incontinence-associated Dermatitis Prevention Practices in an Australian Local Health District: A Quasi-experimental Study

INDUSTRY SPOTLIGHT Industry Spotlight: Graham Dixon, BSc, PhD, CEO and Executive Director, Neem Biotech

SPECIAL REPORT Working with Persons Who Inject Drugs: Be Aware of Wound Botulism

SPECIAL REPORT Wound Care Stakeholders Advocacy Results in Positive Changes in Medicare Coding Process

EMPIRICAL STUDIES A Single-arm, Practical Application Assessment of User Experience and Peristomal Skin Condition Among Persons With an Ileostomy Using a New Barrier Seal With Assisted Flow

EMPIRICAL STUDIES Development of a New Incontinence Containment Product and an Investigation of Its Effect on Perineal Dermatitis in Patients With Fecal Incontinence: A Pilot Study in Women

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Custom Mod Mega1

主任医师、教授、博导，南方医科大学第三附属医院（广东省骨科医院）院长

伤口世界

EMPIRICAL STUDIES Cultivating Incontinence-associated Dermatitis Prevention Practices in an Australian Local Health District: A Quasi-experimental Study

INDUSTRY SPOTLIGHT Industry Spotlight: Graham Dixon, BSc, PhD, CEO and Executive Director, Neem Biotech

SPECIAL REPORT Working with Persons Who Inject Drugs: Be Aware of Wound Botulism

SPECIAL REPORT Wound Care Stakeholders Advocacy Results in Positive Changes in Medicare Coding Process

EMPIRICAL STUDIES A Single-arm, Practical Application Assessment of User Experience and Peristomal Skin Condition Among Persons With an Ileostomy Using a New Barrier Seal With Assisted Flow

EMPIRICAL STUDIES Development of a New Incontinence Containment Product and an Investigation of Its Effect on Perineal Dermatitis in Patients With Fecal Incontinence: A Pilot Study in Women

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