黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
原创:伤口治疗及造口护理中心
一旦有伤口出现,往往会有疼痛的症状发生,这些疼痛反复发作,让许多患者困扰不已。实际上导致患者出现伤口疼痛有些是伤口愈合期间的正常症状,有些由于伤口感染等原因导致,我们应该区别对待,区别处理。
伤口的愈合除了需要良好的环境以外,必不可少的就是需要充足的营养物质的提供。今天小编就给大家介绍一下与伤口愈合紧密相关的一些营养素(分为两次课程)。
原创:伤口治疗及造口护理中心
对于每一个人来说、食物中富含营养素的多少,及其自身吸收利用的程度的高低,将决定着他本人的营养状况。目前对一个人营养状况的评价,大体上是根据我国各类人群中每日膳食营养素供给量的标准米衡量的。对符合每日供给量标准的为营养状况良好,超过的为营养过剩,不足的为营养不良。受历史条件的限制,祖国医学认为人的营养状况取决于饮食结构与进食量是否合理,合理的饮食结构能保障营养素的供给,所以营养状态良好的饮食标准应该是下面几点。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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