Li Yuping,1,7,* Linlin Guan,2 Isabelle Becher,3 Kira S. Makarova,4 Xueli Cao,2 Surabhi Hareendranath,1 Jingwen Guan,1 Frank Stein,3 Siqi Yang,2 Arne Boergel,3 Karine Lapouge,3 Kim Remans,3 David Agard,5 Mikhail Savitski,3 Athanasios Typas,3 Eugene V. Koonin,4 Yue Feng,2,* and Joseph Bondy-Denomy1,6,8,*
1 Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94403, USA
2 State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
3 European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, Germany
4 Computational Biology Branch, Division of Intramural Research, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
5 The Chan-Zuckerberg Institute for Advanced Biological Imaging and the Department of Biochemistry, University of California, San Francisco, San Francisco, CA 94143, USA
6 Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94403, USA
7 Present address: Biozentrum, University of Basel, Basel 4056, Switzerland
8 Lead contact
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (L.Y.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Y.F.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (J.B.-D.)
https://doi.org/10.1016/j.cell.2025.02.016
SUMMARY
Jumbo bacteriophages of the fKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify ‘‘jumbo phage killer’’ (Juk), a two-component immune system that terminates infection of fKZ-like phages, suppressing the expression of early phage genes and preventing phage DNA replication and phage nucleus assembly while saving the cell. JukA (formerly YaaW) rapidly senses the EPI vesicle by binding to an early-expressed phage protein, gp241, and then directly recruits JukB. The JukB effector structurally resembles a pore-forming toxin and destabilizes the EPI vesicle. Functional anti-fKZ JukA homologs are found across bacterial phyla, associated with diverse effectors. These findings reveal a widespread defense system that specifically targets early events executed by fKZ-like jumbo phages prior to phage nucleus assembly.
原创: 十六点五 中山二院糖尿病足中心
如果说大拇指占了人手功能的50%以上的功能,那么足的大趾的功能并没有那么大的作用,实际上,为了追求足底的平面,这三个支撑点的功能都非常重要。
足部对于一个人来说最重要的功能就是站立和行走,这些功能对于一个人来说是非常重要的,没有站立和行走的功能,就意味着这个人无法生活自理,无法工作,需要其他人的照顾,生活质量的急剧下降。
有关糖尿病足这个顽疾,国内外学界最近几年出了很多的指南、专家共识,对于几个比较重要的指南(包括对于2019年版IWGDF糖尿病足指南、2015年版IWGDF糖尿病足指南、中华医学会糖尿病分会糖尿病足及周围血管病变学组等)进行研读之后,有这么几个印象。
2019年版IWGDF有关糖尿病足指南特别推荐的SINBAD系统是指对糖尿病足以下的一些指标进行评价后的分类:Site(位置)、Ischemia(缺血)、Neuropathy(神经病变)、Bacterial infection(细菌感染)、Area(面积)、Depth(深度)。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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