Yuta Tsujisaka,1,2,5 Takeshi Hatani,1,2,5 Chikako Okubo,1 Ryo Ito,1 Azuma Kimura,1 Megumi Narita,1
Kazuhisa Chonabayashi,1,3 Shunsuke Funakoshi,1,4 Antonio Lucena-Cacace,1 Taro Toyoda,1 Kenji Osafune,1
Takeshi Kimura,2 Hirohide Saito,1, * and Yoshinori Yoshida1,4, *
1Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
2Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
3Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
4Takeda-CiRA Joint Program (T-CiRA), Fujisawa 251-0012, Japan
5These authors contributed equally
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.S.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Y.Y.)
https://doi.org/10.1016/j.stemcr.2022.05.003
SUMMARYFor regenerative cell therapies using pluripotent stem cell (PSC)-derived cells, large quantities of purified cells are required. Magnetic-activated cell sorting (MACS) is a powerful approach to collect target antigen-positive cells; however, it remains a challenge to purify various cell types efficiently at large scale without using antibodies specific to the desired cell type. Here we develop a technology that combines microRNA (miRNA)-responsive mRNA switch (miR-switch) with MACS (miR-switch-MACS) to purify large amounts of PSC-derived cells rapidly and effectively. We designed miR-switches that detect specific miRNAs expressed in target cells and controlled the translation of a CD4-coding transgene as a selection marker for MACS. For the large-scale purification of induced PSC-derived cardiomyocytes (iPSC-CMs), we transferred miR-208a-CD4 switch-MACS and obtained purified iPSC-CMs efficiently. Moreover, miR-375-CD4 switch-MACS highly purified pancreatic insulin-producing cells and their progenitors expressing Chromogranin A. Overall, the miR-switch-MACS method can efficiently purify target PSC-derived cells for cell replacement therapy.
白 玲
兰州普瑞眼科医院 甘肃兰州
【摘要】目的 探寻在眼部整形美容术后实行局部冷敷处理的效果。方法 选择我院收治的 60 例眼部整形美容术患者，随机平分成对照组和观察组，分别实行术后常规处理和局部冷敷处理，对比效果。结果 观察组创口开始好转时间（2.11±1.24）、创口基本愈合时间（5.67±2.04）、术眼局部肿胀消退时间（2.04±0.71）明显优于对照组（P＜0.05）；观察组术后创口疼痛程度明显低于对照组（P＜0.05）；观察组 SAS 和 SDS 评分明显低于对照组（P＜0.05），观察组睡眠质量明显高于对照组（P＜0.05）；观察组术后不良反应明显低于对照组（P＜0.05）。结论 局部冷敷处理可使眼部整形美容术后患者的临床各项指标改善，减少疼痛状况，缓解负面情绪，使睡眠质量提升，使不良反应发生率降低。
【关键词】术后；眼部整形美容；局部冷敷处理
【收稿日期】2024 年 2 月 17 日 【出刊日期】2024 年 3 月 25 日 【DOI】10.12208/j.ijcr.20240101
Application of local cold compress treatment in eye plastic surgery
Ling Bai
Lanzhou Purui Eye Hospital, Lanzhou, Gansu
【Abstract】Objective To explore the effect of local cold compress after eye cosmetic surgery. Methods 60 patients in our hospital were randomly divided into control group and observation group, and the postoperative routine treatment and local cold application were applied respectively to compare the effect. Results The observation group (2.11 ± 1.24), basic wound healing time (5.67 ± 2.04), and local swelling regression time (2.04 ± 0.71) were significantly better than controls (P <0.05); significantly lower (P <0.05); SAS and SDS scores were significantly lower (P <0.05) (P <0.05); the observation group was significantly lower than the control group (P <0.05). Conclusion Local cold compress treatment can improve the clinical indicators of patients after eye plastic surgery, reduce the pain status, relieve the negative emotions, improve the sleep quality, and reduce the incidence of adverse reactions.
【Keywords】Postoperative; Eye plastic surgery; Local cold compress treatment
This article is excerpted from the 《Small Science》 by Wound World
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
扫一扫了解详情:
任何关于疾病的建议都不能替代执业医师的面对面诊断。所有门诊时间仅供参考，最终以医院当日公布为准。
网友、医生言论仅代表其个人观点，不代表本站同意其说法，请谨慎参阅，本站不承担由此引起的法律责任。
