Ewa A. BURIAN1, Lubna SABAH1, Klaus KIRKETERP-MØLLER1, Glenn GUNDERSEN2 and Magnus S. ÅGREN1,3,4
1Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark, 2SoftOx Solutions AS, Oslo, Norway, 3Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, and 4Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Yuta Tsujisaka,1,2,5 Takeshi Hatani,1,2,5 Chikako Okubo,1 Ryo Ito,1 Azuma Kimura,1 Megumi Narita,1
Kazuhisa Chonabayashi,1,3 Shunsuke Funakoshi,1,4 Antonio Lucena-Cacace,1 Taro Toyoda,1 Kenji Osafune,1
Takeshi Kimura,2 Hirohide Saito,1, * and Yoshinori Yoshida1,4, *
1Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
2Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
3Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan
4Takeda-CiRA Joint Program (T-CiRA), Fujisawa 251-0012, Japan
5These authors contributed equally
*Correspondence: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (H.S.), 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 (Y.Y.)
https://doi.org/10.1016/j.stemcr.2022.05.003
SUMMARYFor regenerative cell therapies using pluripotent stem cell (PSC)-derived cells, large quantities of purified cells are required. Magnetic-activated cell sorting (MACS) is a powerful approach to collect target antigen-positive cells; however, it remains a challenge to purify various cell types efficiently at large scale without using antibodies specific to the desired cell type. Here we develop a technology that combines microRNA (miRNA)-responsive mRNA switch (miR-switch) with MACS (miR-switch-MACS) to purify large amounts of PSC-derived cells rapidly and effectively. We designed miR-switches that detect specific miRNAs expressed in target cells and controlled the translation of a CD4-coding transgene as a selection marker for MACS. For the large-scale purification of induced PSC-derived cardiomyocytes (iPSC-CMs), we transferred miR-208a-CD4 switch-MACS and obtained purified iPSC-CMs efficiently. Moreover, miR-375-CD4 switch-MACS highly purified pancreatic insulin-producing cells and their progenitors expressing Chromogranin A. Overall, the miR-switch-MACS method can efficiently purify target PSC-derived cells for cell replacement therapy.
原创：DF 中山二院糖尿病足中心
糖尿病足负压引流：（Vacuum Assisted Closure, VAC）负压辅助创面愈合治疗技术是利用生物半透膜使开放创面封闭，使用专用负压机产生一定的负压，通过引流管和敷料作用于清创后的创面，是一种促进急慢性创面愈合的治疗方法。
下肢血管介入手术：糖尿病患者常伴有周围血管病变，尤其下肢动脉血管病变较为突出，是导致糖尿病足溃疡难以愈合的重要因素。
伤口新型敷料的应用，因费用低、效果好、操作方便而被广大患者所接受。
本文原文作者为美国加州大学戴维斯医学中心伤口护理中心主任Holly Kirkland-Kyhn博士，中文译者为河北省唐县白求恩纪念医院的张瑞红老师，作者详细介绍见文末。
伤口世界平台生态圈，以“关爱人间所有伤口患者”为愿景，连接、整合和拓展线上和线下的管理慢性伤口的资源，倡导远程、就近和居家管理慢性伤口，解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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