黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
转载 中诺医疗
“中国新发肝细胞癌(肝癌)占全球55%,中国肝癌居第四,死亡率占第三。”
在6月28日召开的2019中华肿瘤大会上,中国科学院院士、复旦大学附属中山医院院长樊嘉谈到了我国肝癌的现状。①
不仅仅是全球一半以上新发的肝癌在中国,肝癌的致死率也堪忧。
最近《柳叶刀》刊发中国疾病负担研究提到,从1990-2017年之间的中国主要死因中,肝癌一直在第5、6、7位波动,甚至呈上升趋势。②
原创:中诺医疗
世界癌症研究基金会与美国癌症研究所联合发布了第三版《癌症预防和生存报告》。1997年第一版、2007年第二版的《癌症预防和生存报告》曾被誉为是癌症预防科学的里程碑,同时也是癌症预防生活方式最权威和最全面的报告。第三版《癌症预防和生存报告》基于数百项研究的结果、5100万人的数据、350多万的癌症病例。
原创: 十六点五 中山二院糖尿病足中心
糖尿病足的感染是其发病的中心环节,因此,全身抗感染是必须的。问题是在糖尿病足病人用全身抗生素,到底是要达到什么目的?
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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