Ha Young Park a , Min Ho Kang a , Guewha Lee b , Jin Woo Kim a,c,*

a Department of Food Science, Sunmoon University, Chungcheongnam-do, Republic of Korea

b Hu evergreen Pharm Inc., Incheon, Republic of Korea

c Center for Next-Generation Semiconductor Technology, Sun Moon University, Chungnam, Republic of Korea

ARTICLE INFO

Keywords: Ginseng non-edible callus  Extracellular vesicle  Skin regeneration  Collagen synthesis  Proliferation

1 . ABSTRACT

Background: This study aimed to investigate the effects of ginseng non-edible callus-derived extracellular vesicle (GNEV) on skin regeneration, particularly focusing on its impact on proliferation and migration in human dermal fibroblast (HDF).

Methods: GNEV was isolated from ginseng non-edible callus using sequential filtration and size exclusion chromatography (SEC). The extracellular vesicle was characterized using nanoparticle tracking analysis (NTA). HDF was treated with various concentrations of GNEV, and cell viability, proliferation, and migration were assessed using MTT and scratch wound healing assays. Gene expression related to collagen synthesis (TGF-β, SMAD-2, SMAD-3, COL1A1) was measured using RT-PCR.

Results: Treatment of HDF with GNEV resulted in a significant 2.5-fold increase in cell migration compared to the non-treated group. Furthermore, GNEV demonstrated the upregulation of collagen synthesis genes, specifically TGF-β, SMAD-2, SMAD-3, and COL1A1, by 41.7 %, 59.4 %, 60.2 %, and 21.8 %, respectively. These findings indicated that GNEV activates the TGF-β/SMAD signaling pathway, showcasing its potential to induce skin

Conclusions: In conclusion, GNEV exhibits a notable ability to enhance skin regeneration through its stimulatory effects on cell migration and the upregulation of key collagen synthesis genes. The activation of the TGF-β/SMAD signaling pathway further suggests the potential of GNEV as a promising candidate for drug delivery systems in the fields of cosmetics and pharmaceuticals, opening avenues for further research and application in skincare and dermatology

Diala Haykal

Centre Médical Laser Palaiseau, Palaiseau, France

Correspondence: Diala Haykal (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。)

Received: 5 December 2024 | Accepted: 15 December 2024

Funding: The author received no specific funding for this work.

Keywords: cosmetic dermatology | genetic predisposition | personalized skincare | precision medicine | single nucleotide polymorphisms (SNPs) profiling

Liang Chen1,2 | Fudi Wang3 | Xiaoyun Hu1,2 | Nihong Li1,2 | Ying Gao4 | Fengfeng Xue5 | Ling Xie1,2 | Min Xie1,2

1 Scientific Research Laboratory, Shanghai Le-Surely Biotechnology Co. Ltd, Shanghai, China |

2 SASELOMO Research Institute and Biological Laboratory, Shanghai Chuanmei Industrial Co. Ltd, Shanghai, China |

3 Evelab Insight (Singapore) Pte. Ltd, Singapore, Singapore |

4 Zhejiang Moda Biotech Co. Ltd, Hangzhou, China |

5 Nanomedicine and Intestinal Microecology Research Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China

Correspondence: Liang Chen (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。) | Fengfeng Xue (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。)

Received: 5 September 2024 | Revised: 25 December 2024 | Accepted: 24 January 2025

Funding: The authors received no specific funding for this work.

Keywords: antiaging | baicalin | bio-vesicle | skin physiology and cell culture | transdermal delivery

Hawazin Arkan Yousif1 | Israa Al-Ani1 | Maha N. Abu Hajleh2 | Sina Matalqah1 | Wael Abu Dayyih3 |

Emad A. Al-Dujaili4

1 Department of Pharmaceutics and Pharmaceutical Technology, Pharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya

Amman University, Amman, Jordan |

2 Department of Cosmetic Science, Pharmacological and Diagnostic Research Centre, Faculty of Allied Medical

Sciences, Al-Ahliyya Amman University, Amman, Jordan |

3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Al-Karak,

Jordan |

4 Queen's Medical Research Institute, Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK

Correspondence: Maha N. Abu Hajleh (该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。)

Received: 26 August 2024 | Revised: 4 October 2024 | Accepted: 18 November 2024

Keywords: antiaging | coenzyme Q10 | hyaluronic acid | skin care | ubiquinone