黄韶斌 1 胡志成 1 张逸 2 唐冰 1 王鹏 1 徐海琳 1 王志勇 1 董云先 1 程璞 1 荣燕超 1 吴军 3 朱家源 1
1 中山大学附属第一医院烧伤科,广州 510080;
2 南通大学附属医院烧伤整形外科 226001;3 深圳大学第一附属医院烧伤整形科 518037 通信作者:朱家源,Email:该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。
Shaobin Huang1,2† , Zhicheng Hu1† , Peng Wang1 , Yi Zhang3 , Xiaoling Cao1 , Yunxian Dong1 , Pu Cheng1 , Hailin Xu1 ,
Wenkai Zhu4 , Bing Tang1* and Jiayuan Zhu1*
Abstract
Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.
Methods: Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.
Results: The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.
Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Keywords: ESCs, Cell differentiation, Angiogenesis, Full-thickness wounds
原创:十六点五 中山二院糖尿病足中心
糖尿病足的治疗是当今医学界的难题之一,但细细想来,目前糖尿病足的治疗的关键技术好像都不是非常困难,也不是什么高科技,更没有多少“原始创新”。
有些糖尿病人来到中心的糖尿病足工作室,会产生很大的疑问,为什么我的脚明明只有一个很小洞,却在工作室被大大地切开?另外一些患者发现,脚上的一个小小的伤口,几个月甚至几年都不愈合,而另外的一些人却发现,仅仅是剪脚指甲时留下的一个小小的伤口,几天之后自己的脚就变成像猪头一样,到处流脓,而且臭气熏天,苦不堪言。这是为什么?
原创: 十六点五 中山二院糖尿病足中心
随着糖尿病患病人数的不断增加,糖尿病足的病人也越来越多,在平时的临床实践中,笔者发现很多糖尿病足病友搞不清楚糖尿病足应该由什么科管?或者说哪个科的医师负责糖尿病足的诊断和治疗。
控制血糖是预防糖尿病足最重要的措施之一,也是治疗糖尿病足综合治疗的基础之一。当出现严重的并发症糖尿病足的时候,控制血糖最重要的方法目前一般认为是胰岛素治疗,而且最好是胰岛素的强化治疗。但很多患者不是非常理解,往往产生很多问题,对于其中一些有代表性的问题做一些回答。
伤口世界平台生态圈,以“关爱人间所有伤口患者”为愿景,连接、整合和拓展线上和线下的管理慢性伤口的资源,倡导远程、就近和居家管理慢性伤口,解决伤口专家的碎片化时间的价值创造、诊疗经验的裂变复制、和患者的就近、居家和低成本管理慢性伤口的问题。
2019广东省医疗行业协会伤口管理分会年会
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