吕国忠 朱宇刚 周红梅 顾在秋 杨敏杰

[摘要]
目的探讨自.异体表皮细胞悬液混合移植技术在创而修复中的应用、

方法 30只，大鼠随机配成15对后.分成细胞悬液移植组(A组,10对)和细胞膜片移植组(B组,5对)。取每只大鼠的去全厚皮创面分离表皮细胞，并根据配对情况按1:1的细胞比例混合,体外常规培养。4 d后收获A组混合细胞悬液,14 d后收获B组混介细胞膜片、将此细胞悬液和膜片分别转移至A、B组相应供体大鼠的去全厚皮创面、随后A组每对大鼠的创面交叉覆益配对方的体全厚皮;B组创血覆盖胶原膜及“优妥”敷料。比较移梢后2~3周两组的创面修复情况。

结果 术后2~3 尚,A组创面大多愈介,表面光滑.与皮下连接紧密。术后第5天,B组创面部分细胞膜片脱落,部分成活。膜片成活的
创面后期再次出现小创面经久不愈，

结论 自异体表皮细胞悬液混合移植足一种可行的、体内构建皮肤、修复创面的方法。

[关键词] 表皮;细胞培养; 皮肤移植;烧伤

An experimental study on the in vivo intermingled culture of rat autologous and allogeneic epithelial cells LV Cuozhong, ZHU Yugang, ZHOU Hongmei. Department of Burns, The Third People' s Hospital, Wuxi 214041,Jiangsu Province, P. R. China

[ Abstract] Objective To explore a method for in vivo skin reconstruction. Methods Thirty Spraque-Dawley ( SD) rats were randomly divided into two paired groups, i. e. in vivo culture ( A group，10
pairs) and in vitro grafting (B group,5 pairs). Skin samples were harvested from the rats of the two groups for the isolation of epithelial cells which were then mixed cultured in vitro in 1: I ratio. Mixed cellular sus- pension in A group was harvested 4 days after culture. The mixed cellular sheets were harvested 14 days after culture. The cultured cells and sheets were then thansplanted onto ttal skin loss wounds of donor rats for further cultivation. The wounds in A group were covered with allogeneic full-thickness skin. While the wounds in group were covered by collagen membrane and gauze. Wound repair was observed and compared between the two groups al 2-3 post - operative weeks.

Results
Most of the wounds in A group healed after 2-3 weeks with smooth surface, and the peithelium connected closely and tightly with the: subcutaneous tissue. In the wounds in B group on 5 post-operative day . some of the cellular sheets survived and some fell off. Even the healed wounds in B group would be injured again resulting in protracted small wounds.

Conclusion In vivo in situ epithelia! culture might be an optional method of skin reconstruction for wound healing.

[Key words] Epithelium; Cell culture; Skin graft; Burm

朱世琴1 邓波2 李飞2  综述  李显蓉3  审校

1.西南医科大学附属医院内分泌科，四川泸州646000；2.西南医科大学护理学院，四川泸州646000  3. 西南医科大学附属医院胃肠外科，四川 泸州 646000

[摘要]糖尿病足是临床慢性伤口治疗的主要难题之一。负压封闭引流技术作为创面修复领域的一项新技术，目前已被广泛应用于糖尿病足的治疗，并已取得一定的成效。本文将对负压封闭引流技术在糖尿病足治疗及护理中的应用进行相关综述，为罹患糖尿病足的慢性病患者提供一种安全有效的创面修复治疗技术。

[关键词]糖尿病足；封闭负压引流术；护理

[中图分类号]R587.1 [文献标志码] A 10.3969/j.issn.1672-3511.2019.11.033

Application status of vacuum sealing drainage in diabetic foot

ZHU Shiqin1， DENG Bo2， LI Fei2  reviewing  LI Xianrong3 chenking.

[Abstract] Diabetic foot is one of the major problems in clinical chronic wound treatment and it is also an important public health problem. Vacuum sealing drainage technology, as a new technique in the field of wound repair, has been widely used in the treatment of diabetic foot and has achieved certain results. This paper reviews the application of negative pressure sealing drainage technique in treating diabetic foot and nursing care, so as to help clinical nurses grasp accurately the current situation of negative pressure sealing drainage. Thus providing a safe and effective treatment technique for wound healing for patients with chronic diseases suffering from diabetic foot.
[keywords] Diabetic foot ulcers; Vacuum sealing drainage; Nursing

胡葵葵 戴育成 李剑 袁敬东 李洁 吴琼

【摘要】 目的用表皮干细胞和成纤维细胞作为种子细胞，研制一种增殖能力强具有表皮、真皮的组织工程化人工复合皮肤。方法从幼儿包皮中分离表皮干细胞，用胶原Ⅳ纯化、富集表皮干细胞，接种在3T3细胞滋养层上．将体外传代培养的表皮干细胞和真皮成纤维细胞分别接种在经冷冻干燥及戊二醛交联的J．型胶原基质网架的两侧，在液面下培养2周后，改为气一液界面培养，构建复合皮肤，移植到裸鼠全层皮肤缺损处，以表皮细胞胶原海绵复合皮肤和无细胞接种的胶原海绵膜作为对照。术后进行大体观察，在第7、14、21天取材行组织学、免疫组织化学及电镜观察。结果用表皮干细胞构建的复合人工皮肤增殖能力强．新生的皮肤瘢痕轻，形态满意，创面愈合的速度和质量均优于对照组。结论在胶原海绵上用表皮干细胞构建的复合人工皮肤增殖能力强，较好地解决了种子细胞的老化问题，可望成为一种较为理想的皮肤替代物。

【关键词】 表皮干细胞； 复合人工皮肤； 创面愈合； 组织工程

Repair of fu¨skin Ioss with composite graft of epidermal stem cells in nude mice HU Kui-kui，DAI Yu一cheng，Li Jian ，YUAN Jing—dong，Li Jie，WU Qiong．Center of Plastic，laser and  Cosmetic surger一y，Second Affiliated Hospital, Jiangxi Medical Collage , Nanchang   330006，China

【Abstract】

Objective: To constitute a composite skin substitute that can proliferate well with epidermal stem cells and fibroblasts on collagen sponge．

Methods:  Epidermal stem cells were selected by rapid attachment to c01lagenⅣfor 10～15 min and cultured on 3T3 feeder layer． Collagen was extracted from rat tail_ The matrix lattice was fabricated by a freeze—dryer and cross—linked with glutaraldehyde． Fibroblasts (1 x 106／cm2) were inoculated on collagen sponge and cultured for 2 weeks prior to inoculation of epidermal stem cells(2×105／cm2) to construct composite skin substitute．The artificial skin was grafted onto full skin 10ss wounds of nude mice．Collagen sponge membrane lacking cell inoculation and a composite skin substitute with keratinocytes and fibroblasts were used as controls． The wounds were observed daily． Tj\issue samples were harvested and examined by means of histology，immunohistochemistry and electron microcopy．

Results：Composite graft with epidermal stem cells proliferated promptly，and achieved a good adherence to wound on the 3rd day of grafting．the mice exhibited less epidermal scar formation and satisfactory contour of the skin．The quality and speed of wound healing in experimental groups were superior to those in the control groups．

Conclusion: Composite graft with epidermal stem cells have potential prospects in application of repairing skin defect with advantage of good proliferation．

【Key words】Epidermal stem cell；Compound artificial skin；Wound healing；Tissue engineerlng