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【摘 要】 目的 观察脉复生联合杏芎氯化钠注射液治疗下肢动脉硬化闭塞症患者的临床疗效。方法 将 206 例下肢动脉硬化闭塞症患者随机分为治疗组和对照组各 103 例。两组均在常规治疗基础上给予杏芎氯化钠注射液
( 100 mL 静滴，qd) ，治疗组加服用脉复生( 30 mL，bid) ，治疗 15 d 后，观察治疗前后患者创面愈合率、疼痛程度 VAS 评分、血脂水平、血液流变学、患肢经皮氧负压( TcPO2 ) 、踝肱指数( ABI) 的变化。结果 治疗组总有效率为 96. 11% ，显著高于对照组的 77. 1% ( P ＜ 0. 05) ; 治疗组疼痛减轻程度大于对照组( P ＜ 0. 05) ，经皮氧负压高于对照组( P ＜ 0. 05) 。结论 脉复生联合杏芎氯化钠注射液治疗下肢动脉硬化闭塞症患者有显著疗效，有助于减轻患者溃疡疼痛、提高患肢经皮氧分压、促进患肢溃疡愈合。
【关键词】 下肢动脉硬化闭塞症; 脉复生; 杏芎氯化钠注射液; 下肢溃疡; 经皮氧分压 ABI
【摘 要】目的: 调查肠造口患者病耻感与出院准备度现状，并探讨两者相关性。方法: 选取北京协和医院结直肠外科行肠造口术住院患者 124 例，采用出院准备度量表 ( RHDS) 评价出院准备度状况，用社会影响量表 ( SIS) 评价病耻感状况。结果: 居住在农村的患者 SIS 得分高于居住在城市的患者，家庭月均收入≤3000 元的患者 SIS 高于家庭月均收入 ＞ 3000 元的患者 ( 均 P ＜ 0. 05) 。患者 SIS 总分及各维度得分均与 RHDS 总分呈负相关 ( r = － 0. 35、 － 0. 31、 － 0. 26、 － 0. 32、 － 0. 36，均 P ＜ 0. 01) 。多重回归分析显示 SIS 的内在羞耻感维度得分与 RHDS 总分负向关联 ( β = － 3. 33，P ＜ 0. 05) 。结论: 肠造口患者病耻感与出院准备度密切相关。
【关键词】肠造口; 病耻感; 出院准备度
Background: Glycolysis dysfunction is an important pathogenesis of podocyte injury in diabetic kidney disease (DKD). Foot process fusion of podocytes and increased albuminuria are markers of early DKD. Moreover, cytoskeletal remodeling has been found to be involved in the foot process fusion of podocytes. However, the connections between cytoskeletal remodeling and alterations of glycolysis in podocytes in DKD have not been clarifified.
Methods: mRNA sequencing of glomeruli obtained from db/db and db/m mice with albuminuria was performed to analyze the expression profifiling of genes in glucose metabolism. Expressions of phosphofructokinase platelet type (PFKP) in the glomeruli of DKD patients were detected. Clotrimazole (CTZ) was used to explore the renal effects of PFKP inhibition in diabetic mice. Using Pfkp siRNA or recombinant plasmid to manipulate PFKP expression, the effects of PFKP on high glucose (HG) induced podocyte damage were assessed in vitro. The levels of fructose-1,6-bisphosphate (FBP) were measured. Targeted metabolomics was performed to observe the alterations of the metabolites in glucose metabolism after HG stimulation. Furthermore, aldolase type b (Aldob) siRNA or recombinant plasmid were applied to evaluate the inflfluence of FBP level alteration on podocytes. FBP was directly added to podocyte culture media. Db/db mice were treated with FBP to investigate its effects on their kidney.
Results: mRNA sequencing showed that glycolysis enzyme genes were altered, characterized by upregulation of upstream genes (Hk1, and Pfkp) and down-regulation of downstream genes of glycolysis (Pkm, and Ldha). Moreover, the expression of PFKP was increased in glomeruli of DKD patients. The CTZ group presented more severe renal damage In vitro, the Pfkp siRNA group and ALDOB overexpression group showed much more induced cytoskeletal remodeling in podocytes, while overexpression of PFKP and suppression of ALDOB in vitro rescued podocytes from cytoskeletal remodeling through regulation of FBP levels and inhibition of the RhoA/ROCK1 pathway. Furthermore, targeted metabolomics showed FBP level was signifificantly increased in HG group compared with the control group. Exogenous FBP addition reduced podocyte cytoskeletal remodeling and renal damage of db/db mice.
Conclusions: These fifindings provide evidence that PFKP may be a potential target for podocyte injury in DN and provide a rationale for applying podocyte glycolysis enhancing agents in patients with DKD.
Keywords: PFKP, diabetic kidney diseases, glycolysis, podocyte injury, cytoskeletal remodeling, FBP
ABSTRACT
Introduction: The therapeutic effect of chemical lumbar sympathectomy (CLS) on ischemic diseases of the lower limbs varies greatly among individuals. The time to peak (TTP) response in contrast-enhanced ultrasound (CEUS) can reflflect the perfusion disorder of the calf skeletal muscle and the collateral circulation. In this study we evaluated the predictive value of CEUS in patients treated with CLS for end-stage atherosclerotic occlusive disease of the lower extremity (ASO-LE).
Methods: This was a prospective study that included patients with end-stage ASO-LE and moderate to severe pain who had undergone a CEUS examination and CLS procedure and who were observed for 12 months after surgery. The patients’ characteristics and prognostic factors,including lower limb pain score, skin temperature, walking distance, and ulcer and gangrene healing, were recorded.
Results: Fifty-eight patients with a mean age of 66.24 (range 58–78) years were included in the study, of whom 42 (71.41%) were men. Following the CLS procedure, the numerical rating scale (NRS)-measured pain decreased signififi- cantly, and the skin temperature of the affected limb increased signifificantly (P \0.05). The satisfaction rate of lower limb pain relief 1 year after operation was 53.45%. Correlation analysis showed that preoperative TTP response was correlated with the NRS score and skin temperature of the affected limb at 6 months and 12 months post surgery (P \ 0.05). The binary logistic regression analysis indicated that a longer preoperative TTP response was associated with a higher risk of poor pain relief after CLS (odds ratio 1.126, 95% confifidence interval 1.058–1.205). The receiver operating characteristic curve showed that preoperative TTP response had a certain predictive value on CLS treatment effect, with a sensitivity and speci-fificity of 81.5% and 83.9%, respectively. When the preoperative TTP response was [ 77.5, the risk of poor response after CLS increased.
Conclusions: Preoperative TTP response was able to predict the therapeutic effect of CLS to a certain extent, and thus may aid physicians in determining the choice of CLS treatment for patients with ASO-LE.
Trial Registration: Chinese Clinical Trial Registry: ChicTR1900028424 (principal investigator: Yue Wu; date of registration: 21 December 2019).
Keywords: The time to peak; Peripheral arterial disease; Arteriosclerosis obliterans; Skeletal muscle; Collateral circulation
